Construction and Primary Analysis of Suppression Subtractive Hybridization Libraries for Female Apis cerana

FAN Ying; ZOU Fengcai; DONG Xia; KUANG Haiou

doi:10.16211/j.issn.1004-390X(n).2015.05.010

JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science） > 2015 > 30(5): 710-721. > DOI: 10.16211/j.issn.1004-390X(n).2015.05.010

FAN Ying, ZOU Fengcai, DONG Xia, et al. Construction and Primary Analysis of Suppression Subtractive Hybridization Libraries for Female Apis cerana[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2015, 30(5): 710-721. DOI: 10.16211/j.issn.1004-390X(n).2015.05.010

Citation:

PDF (1296 KB)

Construction and Primary Analysis of Suppression Subtractive Hybridization Libraries for Female Apis cerana

1.
1. Eastern Bee Research Institute, Yunnan Agricultural University, Kunming 650201, China
2.
2. The Livestock or Poultry Genetic Resource of Management Station, Guiyang 553000, China
3.
4.
College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China
5.
Eastern Bee Research Institute, Yunnan Agricultural University, Kunming 650201, China

More Information

Received Date: February 08, 2015
Revised Date: May 07, 2015
Published Date: September 15, 2015

Graphical Abstract

Abstract

Abstract

The two different phenotypes of honeybees, queen and workers, are determined by the differences of gene expressions rather than the polymorphisms of the genetic material. The aim was to construct differential gene expression libraries by suppression subtractive hybridization (SSH) for queen and worker bees of Apis cerana.We obtained 161 and 142 high quality ESTs sequences form queen and worker bees,respectively.After using DNAstar seqMan for carriers and joints removing,low quality sequence filtering,and ESTs clustering,we finally obtained 110 and 81 contings for queen and worker bees subtracted libraries,respectively.There are overlapping ESTs sequences for both queen and worker bees subtracted libraries. Using sequence homology analysis by BLASTx and BLASTn,62 out of 65 ESTs from the queen subtracted library and 49 out of 57 ESTs from the worker subtracted library are found to have homologous genes,respectively. Gene function analysis by Gene Ontology (GO) suggested that the products of majority of the differentially expressed genes are enzymes and binding protein, which are involved in gene transcription,translation and metabolism process.The female A.cerana subtracted libraries constructed in our study provide important information for the further studies of the mechanism of caste determination.
- Apis cerana,
- suppression subtractive hybridization(SSH),
- different express gene,
- subtracted library,
- ESTs

FullText(HTML)

References (20)

References

[1]	匡邦郁, 匡海鸥.蜜蜂生物学[M].昆明: 云南科技出版社, 2003.
[2]	[2] 张复兴.现代养蜂生产[M].北京: 中国农业大学出版社, 1998.
[3]	[3] 曾志将.蜜蜂生物学[M].北京: 中国农业出版社, 2007.
[4]	[4] Graham J M.The hive and the honey bee[M]. Illinois: Dadant Sons, INC., 1992
[5]	[5] HARTFELDER K, ENGELS W. Social insect polymorphism: Hormonal regulation of plasticity in development and reproduction in the honeybee[J]. Current Topics in Developmental Biology, 1998, 40: 45-77.
[6]	[6] SEVERSON D W, WILLIAMSON J L, AIKEN J M. Caste-specific transcription in the female honey bee[J]. Insect Biochemistry, 1989, 19(2): 215-220.
[7]	[7] CORONA M, ESTRADA E, ZURITA M.Differential expression of mitochondrial genes between Queens and workers during caste determination in the honeybee Apis mellifera[J]. The Journal of Experimental Biology, 1999, 202(Pt 8): 929-938.
[8]	[8] EVANS J D, WHEELER D E.Differential gene expression between developing Queens and workers in the honey bee, Apis mellifera[J].Proceedings of the National Academy of Sciences of the United States of America, 1999, 96(10): 5575-5580.
[9]	[9] CRISTINO A S, NUNES F, LOBO C H, et al..Caste development and reproduction: A genome-wide analysis of hallmarks of insect eusociality[J]. Insect Molecular Biology, 2006, 15(5): 703-714.
[10]	[10] DONNELL D M, STRAND M R. Caste-based differences in gene expression in the polyembryonic wasp Copidosoma floridanum[J]. Insect Biochemistry and Molecular Biology, 2006, 36(2): 141-153.
[11]	[11] BARCHUK A R, CRISTINO A S, KUCHARSKI R, et al..Molecular determinants of caste differentiation in the highly eusocial honeybee Apis mellifera[J].BMC Developmental Biology, 2007, 7(1): 70.
[12]	[12] KUCHARSKI R, MALESZKA J, FORET S, et al..Nutritional control of reproductive status in honeybees via DNA methylation[J].Science, 2008, 319(5871): 1827-1830.
[13]	[13] ZHANG Yi, LIU Xuejiao, ZHANG Wenqing, et al..Differential gene expression of the honey bees Apis mellifera and A.cerana induced by Varroa destructor infection[J].Journal of Insect Physiology, 2010, 56(9): 1207-1218.
[14]	[14] DIATCHENKO L, LAU Y F, CAMPBELL A P, et al..Suppression subtractive hybridization: A method for generating differentially regulated or tissue-specific cDNA probes and libraries[J].Proceedings of the National Academy of Sciences of the United States of America, 1996, 93(12): 6025-6030.
[15]	[15] 蔡小辉, 鲁义善, 吴灶和, 等.溶藻弧菌诱导凡纳滨对虾消减文库的构建及其表达序列标签分析[J].水生生物学报, 2010(1): 157-163.
[16]	[16] JAVID B, MACARY P A, LEHNER P J.Structure and function: Heat shock proteins and adaptive immunity[J]. Journal of Immunology, 2007, 179(4): 2035-2040.
[17]	[17] CUNHA A D, NASCIMENTO A M, GUIDUGLI K R, et al..Molecular cloning and expression of a hexamerin cDNA from the honey bee, Apis mellifera[J].Journal of Insect Physiology, 2005, 51(10): 1135-1147.
[18]	[18] DANTY E, ARNOLD G, BURMESTER T, et al..Identification and developmental profiles of hexamerins in antenna and hemolymph of the honeybee, Apis mellifera[J].Insect Biochemistry and Molecular Biology, 1998, 28(5/6): 387-397.
[19]	[19] ZHOU Xuguo, TARVER M R, SCHARF M E.Hexamerin-based regulation of juvenile hormone-dependent gene expression underlies phenotypic plasticity in a social insect[J].Development, 2007, 134(3): 601-610.
[20]	[20] BITONDI M M, NASCIMENTO A M, CUNHA A D, et al..Characterization and expression of the Hex 110 gene encoding a glutamine-rich hexamerin in the honey bee, Apis mellifera[J].Archives of Insect Biochemistry and Physiology, 2006, 63(2): 57-72.

Cited By

Get Citation

PDF

XML

Article views (1981) PDF downloads (481)

Turn off MathJax

Article Contents

Abstract

References

Construction and Primary Analysis of Suppression Subtractive Hybridization Libraries for Female Apis cerana

Abstract

References

Catalog

Export File

Citation

Format

Content