Construction and Primary Analysis of Suppression Subtractive Hybridization Libraries for Female Apis cerana

FAN Ying; ZOU Fengcai; DONG Xia; KUANG Haiou

doi:10.16211/j.issn.1004-390X(n).2015.05.010

JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science） > 2015 > 30(5): 710-721. > DOI: 10.16211/j.issn.1004-390X(n).2015.05.010

FAN Ying, ZOU Fengcai, DONG Xia, et al. Construction and Primary Analysis of Suppression Subtractive Hybridization Libraries for Female Apis cerana[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2015, 30(5): 710-721. DOI: 10.16211/j.issn.1004-390X(n).2015.05.010

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Construction and Primary Analysis of Suppression Subtractive Hybridization Libraries for Female Apis cerana

1.
1. Eastern Bee Research Institute, Yunnan Agricultural University, Kunming 650201, China
2.
2. The Livestock or Poultry Genetic Resource of Management Station, Guiyang 553000, China
3.
4.
College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China
5.
Eastern Bee Research Institute, Yunnan Agricultural University, Kunming 650201, China

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Received Date: February 08, 2015
Revised Date: May 07, 2015
Published Date: September 15, 2015

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Abstract

The two different phenotypes of honeybees, queen and workers, are determined by the differences of gene expressions rather than the polymorphisms of the genetic material. The aim was to construct differential gene expression libraries by suppression subtractive hybridization (SSH) for queen and worker bees of Apis cerana.We obtained 161 and 142 high quality ESTs sequences form queen and worker bees,respectively.After using DNAstar seqMan for carriers and joints removing,low quality sequence filtering,and ESTs clustering,we finally obtained 110 and 81 contings for queen and worker bees subtracted libraries,respectively.There are overlapping ESTs sequences for both queen and worker bees subtracted libraries. Using sequence homology analysis by BLASTx and BLASTn,62 out of 65 ESTs from the queen subtracted library and 49 out of 57 ESTs from the worker subtracted library are found to have homologous genes,respectively. Gene function analysis by Gene Ontology (GO) suggested that the products of majority of the differentially expressed genes are enzymes and binding protein, which are involved in gene transcription,translation and metabolism process.The female A.cerana subtracted libraries constructed in our study provide important information for the further studies of the mechanism of caste determination.
- Apis cerana,
- suppression subtractive hybridization(SSH),
- different express gene,
- subtracted library,
- ESTs

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