The Marker Gene ACO of Fruits Preservation

Fruits preservation and fructescence were important factors that influenced the economic value of fruits. ACC Oxidase (ACO) was an important enzyme and played a crucial role in regulating fruit growth. To study the mechanism that ACO catalyzed ACC to ethylene might be contributed to mark and improve fruits preservation. Fruit ACO genes data was derived from Actinidia chinensis, Actinidia deliciosa, Vitis vinifera, Malus domestica, Pyrus communis, Pyrus pyrifolia, Prunus persica, Prunus mume, Prunus armeniaca, and Musa ABB. Several aspects of bioinformatics analysis, such as amino acids sequences analysis and chemical properties analysis and phylogenic prediction of them, were conducted. Those fruit trees were grouped together by cluster analysis based on the bioinformatics analysis results. The results of this study were as follows: (1)The fruits preservation of M.domestica was longer than P.mume, P.persica and P.armeniaca had the equal fruit preservation capability. P.communis and P.pyrifolia had the equal fruit preservation capability. A.chinensis and A.deliciosa had the equal fruit preservation capability. (2)According to the fruits preservation, the plants in a common family could be clustered together by the ACO genes. This might be related to variations on the 18th locus in ACO protein sequence, and highly conserved regions in the 2OG-FeII Oxy superfamily, and ACO mechanism affected by several factors.(3)The mechanism of ACO that the hydrophilic ACO protein synthesized by free ribosomes, directly catalyzed ACC to ethylene in the membrane surface, might be affected by genotype and the outside stimulation sensitivity. The ACO gene was an important function gene: the ACO genes could mark the fruits preservation of the plants among common genus.