The Results Observe of Induced by HMC Toxin Treatment in Maize Seedling Root Cap Cell Apoptosis

To study the feature of apoptosis and the change of its ratio induced by HMC toxin in the root cap cells detached from two pair of homokaryon C and N inbred line of maize. Acridine orange (AO)and ethidium bromide (EB) staining methods were used to detach the apoptosis of root cap cells. In the process of induction, root cap cells appeared apoptotic bodies and marginate of chromosome morphology. Living, necrosis and apoptosis cells could be distinguished clearly by AO and EB complex staining. The result showed that the ratio of apoptosis in C cells was sharply higher than that of N, and the max ratios of apoptosis of B37-C and B37-N stained by AO and EB were 45.3% and 15.3%, respectively. In contrast, the max ratios of apoptosis of Mo17-C and Mo17-N stained by AO and EB were 63.7% and 6.39%, respectively. It is concluded that harmed by HMC toxin, the root cap cells appear apoptosis. The ratio of apoptosis in C cytoplasm was higher than in N cytoplasm. Along with the increase of toxin concentration and treating time, the ratio of apoptosis became higher. HMC toxin induced root cap cells apoptosis, cell nucleus issued a green fluorescent, necrotic nuclei are orange red fluorescence, and loose granular cell nucleus or crescent, apoptotic cells emit yellow or green fluorescence, chromosome becomes pyknotic crescent or granule shape.