Study on in vitro Propagation of Rosa sterilis

The young stem was taken as the explants for studying on tissue culture of Rosa sterilis. The factors of tissue culture were studied, including the disinfection of explants, the selection of optimum consistency and combination of hormones, the selection of optimum culture medium combinations of root induction and the transplant of the tissue culture seedling. The results showed that the optimum explants sterilization time was dip in 0.1% HgCl2 for 12 min, and the effective survival rate was 77.78%. The suitable combination for the inducement of axillary buds was MS+6-BA 0.5 mg/L+IBA 0.1 mg/L, and germination rate was 100%. Shoots cultured on the medium of MS+6-BA 0.2 mg/L +IBA 0.02 mg/L could be multiplicated by 3.22 fold and above. The media formula for rooting was 1/2 MS +IBA 0.3 mg/L.In this media, the rootage rate was 100%, and the survival rate was 95% after transplanting.