Purpose To explore the effects of low phosphorus on the growth and enzyme activities of different oat varieties and to screen for low phosphorus-tolerant oat varieties.
Methods Using 15 oat varieties as materials, two treatments of normal phosphorus (1.00 mmol/L) and low phosphorus (0.02 mmol/L) were set up, and they were cultivated in a climate-controlled chamber on perlite substrate for 30 days. The height of the plant, the length and width of the flag leaf were measured with a tape measure, root growth indicators were measured with a root scanner, phosphorus content in the aboveground and underground parts of the plants was determined using the molybdenum-antimony anti-colorimetric method, and enzyme activity was measured using a kit. Finally, a grey relational analysis was used to comprehensively evaluate the above indicators under low phosphorus treatment.
Results Compared with the normal phosphorus treatment, the low phosphorus treatment significantly reduced the plant height of 11 oat varieties, the flag leaf length of 13 varieties and the flag leaf width of 14 varieties; while the root length of 14 oat varieties, the root system projection area and root surface area of 10 varieties, the root volume of nine varieties, as well as the root tip number and root bifurcation number of 12 varieties significantly increased; under low phosphorus treatment, the activities of peroxidase, superoxide dismutase and acid phosphatase, as well as malondialdehyde content, significantly increased in most oat varieties, while the catalase activity, and the total phosphorus content in roots and leaves, significantly decreased.
Conclusion The preliminary study screened out Menglong and Adremo as low phosphorus-tolerant varieties, while Morgon and Longyan No. 5 were identified as low phosphorus-sensitive varieties.
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