Genome-wide Identification and Expression Pattern of PGIP Gene Family in Gentiana rigescens

Purpose To explore the potential function of the polygalacturonase-inhibiting protein (PGIP) gene in the corolla temperature sensing movement of Gentiana rigescens.

Methods Based on the genome-wide and transcriptome data of G. rigescens, bioinformatics methods were used to analyze the physicochemical properties, gene structure, phylogenetic relationships, conserved structural domains, cis-acting elements and expression patterns of the PGIP gene family.

Results The GrPGIP gene family contained 10 members, distributing unevenly on the chromosomes 1, 2, 4, 5, and 9. The GrPGIP genes all contained exons. GrPGIP were all hydrophilic proteins, and subcellular localisation predicted that they were located predominantly in the cell wall. The promoter regions of most GrPGIP gene family members contained light-responsive elements and hormone-responsive elements. Colinearity analysis showed that the PGIP gene family was conserved in dicotyledonous plants, and GO enrichment analysis indicated that the GrPGIP genes might play important roles in the cell wall. 10 GrPGIP genes were differentially expressed during the corolla temperature sensing movement in G. rigescens, which suggested that the GrPGIP genes might be involved in the corolla movement.

Conclusion The results of the study preliminarily elucidate the expression pattern of GrPGIP during the corolla temperature sensing movement, laying the foundation for further research on the function of GrPGIP in the corolla temperature sensing movement in G. rigescens.