Purpose To investigate the effects of adding fermented feed to diet on the growth performance, police performance and intestinal health of Kunming dog puppies.
Methods Ten 62-64 days old Kunming dog puppies were selected and randomly divided into control group (CG) and experimental group (EG). CG was fed with basal diet, EG was fed with 40% of basal diet+60% of fermented feed, and both of them were fed for 28 days. During the experiment period, growth performance and diarrhea rate of the two groups were determined; fecal samples were collected on the 28th day for 16S rDNA sequencing; and on the 22nd and 29th day, the police performance was scored.
Results Compared with CG, the average daily gain of EG was significantly increased (P<0.05), and the feed-to-gain ratio and diarrhea rate were significantly decreased (P<0.05); the police performance scores of EG were significantly higher (P<0.05) on the 22nd and 29th day. The ACE and Shannon indices of EG were significantly higher (P<0.05); at the phylum level, the relative abundance of Fusobacteriota was decreased, and the relative abundance of Proteobacteria was increased (P<0.05); at the genus level, the relative abundance of Limosilactobacillus, Lactobacillus, Streptococcus and Phascolarctobacterium was significantly increased (P<0.05), and part of them were positively correlated with police performance (P<0.05).
Conclusions Fermented feed can optimize the intestinal flora structure of Kunming dog puppies, improving intestinal health, reducing diarrhea rate, and improving growth performance and police performance.
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