Purpose To investigate the effects of different additions of Lycium barbarum residue in diet on delayed-type hypersensitivity, xylene-induced ear allergy, and egg white-induced footpad inflammation in mice.
Methods A total of 200 Kunming mice (21-day-old) were randomly divided into five groups, with 40 mice in each group. Group Ⅰ was the control group (without L. barbarum residue in diet), and groups Ⅱ, Ⅲ, Ⅳ and Ⅴ were fed diets containing 2%, 4%, 6% and 8% L. barbarum residue, respectively. The mice were subjected to delayed-type hypersensitivity test, xylene-induced ear allergic swelling test and egg white-induced footpad inflammation test.
Results Compared with the control group, the right hind footpads of mice in groups Ⅲ, Ⅳ and Ⅴ showed significant swelling after injection of 10% chicken erythrocyte suspension for one hour; after injection for 24 hours, all experimental groups showed significant swelling, among them, group Ⅳ showing the most significant swelling. In the xylene-induced ear swelling test, the ear swelling degree of male mice was higher than that of female mice. Feeding diets containing L. barbarum residue could significantly reduce ear swelling, with group Ⅳ showing the most significant improvement. One hour after egg white injection, the footpad swelling of mice was most obvious, and it basically subsided after four hours. The footpad swelling subsided more significantly in groups Ⅲ and Ⅳ.
Conclusion Adding different contents of L. barbarum residue to the diet of mice can promote the response of mice to delayed-type hypersensitivity, xylene-induced ear allergic swelling and egg white-induced footpad inflammation, that is, effectively reduce inflammation and allergic reactions in mice, indicating that L. barbarum residue has the functions of enhancing cellular immunity, anti-inflammation, and anti-allergy.
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