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| Citation: |
LI Bo, JIAO Jianlin, LUO Ting, et al. Comparative Study of Induced Parkinson’s Disease Model in Tree Shrew by MPTP Subcutaneous and Intramuscular Injections[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2024, 39(4): 45-53. DOI: 10.12101/j.issn.1004-390X(n).202405041
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To establish an ideal animal model of Parkinson’s disease (PD) in tree shrews, subcutaneous and intramuscular injections of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) were used to observe the motor symptoms and brain histopathological changes in the tree shrews.
The tree shrew PD model was induced by subcutaneous and intramuscular injections of 2 mg/kg MPTP, and the motor symptoms of PD were observed. A behavioral rating scale assessing tremor, posture, locomotion, gait, and balance was established for the tree shrew PD model with reference to the quantitative assessment criteria for Parkinson’s motor symptoms in non-human primates and patients; morphological changes in the midbrain of the tree shrews were observed by immunohistochemical staining of tyrosine hydroxylase (TH) and α-synuclein (α-syn). mRNA expression of TH and α-syn in the brain tissues of the tree shrew PD model was analyzed by qPCR.
Typical motor symptoms of PD appeared after the 3rd injection in the two injection methods, and the most obvious PD motor symptoms were found after the 4th injection, which were obviously weakened after the 7th injection and basically disappeared in the 15th injection. Immunohistochemical staining of TH and α-syn revealed that there was a decrease in the number of TH-positive nerve fibers in the substantia nigra and an abnormal increase in the number of α-syn in both the subcutaneous and intramuscular injections groups, demonstrating typical PD pathology. In the two injections groups, TH mRNA expression in the brain tissues was extremely significantly decreased and α-syn mRNA expression was extremely significantly increased.
Repeated subcutaneous and intramuscular injections of 2 mg/kg MPTP both can induce consistent motor symptoms and lesions typical of PD in tree shrews, and both can be used to model PD in tree shrews.
| [1] |
SONG Z Y, LIU S, LI X Y, et al. Prevalence of Parkinson’s disease in adults aged 65 years and older in China: a multicenter population-based survey[J]. Neuroepidemiology, 2022, 56(1): 50. DOI: 10.1159/000520726.
|
| [2] |
贾子瑞, 马泽宇, 秦齐, 等. 肠道菌群在帕金森病发病中的作用及机制研究进展[J]. 河南大学学报(医学版), 2020, 39(4): 287. DOI: 10.15991/j.cnki.41-1361/r.2020. 04.016.
|
| [3] |
王肖龙. 内科学[M]. 上海: 上海科学技术出版社, 2020.
|
| [4] |
蒙健林, 梁健芬, 张兴博, 等. 帕金森病实验动物模型的研究进展及评价[J]. 中国实验动物学报, 2021, 29(3): 399. DOI: 10.3969/j.issn.1005-4847.2021.03.016.
|
| [5] |
JOERS V, MASILAMONI G, KEMPF D, et al. Microglia, inflammation and gut microbiota responses in a progressive monkey model of Parkinson’s disease: a case series[J]. Neurobiology of Disease, 2020, 144: 105027. DOI: 10.1016/j.nbd.2020.105027.
|
| [6] |
史良琴, 罗启慧, 曾文, 等. MPTP诱导慢性帕金森病恒河猴模型的初步建立[J]. 浙江大学学报(农业与生命科学版), 2014, 40(3): 257. DOI: 10.3785/j.issn.1008-9209.2013.10.281.
|
| [7] |
朱岳峰, 董乐, 樊晶鑫, 等. 皮下注射MPTP诱导猕猴双侧慢性帕金森病模型的制作[J]. 中国临床神经外科杂志, 2014, 19(12): 735. DOI: 10.13798/j.issn.1009-153X.2014.12.010.
|
| [8] |
胡亚松. MPTP诱导的食蟹猴慢性偏侧帕金森病模型行为学评价研究[D]. 南宁: 广西医科大学, 2017.
|
| [9] |
张志成, 宋庆凯, 黎晓慧, 等. MPTP诱导树鼩帕金森疾病模型行为学检测方法研究[J]. 实验动物与比较医学, 2019, 39(1): 9. DOI: 10.3969/j.issn.1674-5817.2019.01.0 03.
|
| [10] |
邓苙, 邬继兰, 戴萍, 等. 树鼩帕金森病(PD)模型的建立及对其的行为学和形态学研究[J]. 神经解剖学杂志, 2017, 33(3): 341. DOI: 10.16557/j.cnki.1000-7547.2017.03.015.
|
| [11] |
MA K L, GAO J H, HUANG Z Q, et al. Motor function in MPTP-treated tree shrews (Tupaia belangeri chinensis)[J]. Neurochemical Research, 2013, 38: 1935. DOI: 10.1007/s11064-013-1099-8.
|
| [12] |
林臻, 陈洪志, 赵航, 等. MPTP诱导C57BL/6小鼠帕金森模型的制备和评估[J]. 中国比较医学杂志, 2020, 30(8): 57. DOI: 10.3969/j.issn.1671-7856.2020.08.009.
|
| [13] |
SANTORO M, FADDA P, KLEPHAN K J, et al. Neurochemical, histological, and behavioral profiling of the acute, sub-acute, and chronic MPTP mouse model of Parkinson’s disease[J]. Journal of Neurochemistry, 2023, 164(2): 121. DOI: 10.1111/jnc.15699.
|
| [14] |
曾朝蓉, 岳冬, 孙伟, 等. MPTP诱导帕金森模型大鼠的血液学及临床病理评价[J]. 成都医学院学报, 2020, 15(1): 23. DOI: 10.3969/j.issn.1674-2257.2020.01.005.
|
| [15] |
李春艳. 菊苣酸对1-甲基-4-苯基-1,2,3,6-四氢吡啶诱导斑马鱼帕金森病模型的神经保护作用[D]. 烟台: 烟台大学, 2023.
|
| [16] |
陈生弟, 周孝达, 唐琴梅, 等. 应用MPTP制备猫帕金森病动物模型: 行为、生化和组织学研究[J]. 中国神经精神疾病杂志, 1992(4): 225.
|
| [17] |
解洪荣, 张玉花, 李雪松, 等. MPTP/MPP+诱导的帕金森病实验模型的研究进展[J]. 医学综述, 2011, 17(5): 669. DOI: 10.3969/j.issn.1006-2084.2011.05.011.
|
| [18] |
FAN Y, YE M S, ZHANG J Y, et al. Chromosomal level assembly and population sequencing of the Chinese tree shrew genome[J]. Zoological Research, 2019, 40(6): 506. DOI: 10.24272/j.issn.2095-8137.2019.063.
|
| [19] |
FAN Y, HUANG Z Y, CAO C C, et al. Genome of the Chinese tree shrew[J]. Nature Communications, 2013, 4: 1426. DOI: 10.1038/ncomms2416.
|
| [20] |
郭玉倩. 人参皂苷Rg1通过Wnt信号通路改善阿尔茨海默病树鼩认知障碍[D]. 昆明: 昆明医科大学, 2020.
|
| [21] |
LI H, XIANG B L, LI X, et al. Cognitive deficits and Alzheimer’s disease-like pathologies in the aged Chinese tree shrew[J]. Molecular Neurobiology, 2024, 61(4): 1892. DOI: 10.1007/s12035-023-03663-7.
|
| [22] |
SHEN F, QI K K, DUAN Y, et al. Differential effects of clomipramine on depression-like behaviors induced by the chronic social defeat paradigm in tree shrews[J]. Journal of Psychopharmacology, 2018, 32(10): 1141. DOI: 10.1177/0269881118793560.
|
| [23] |
SUN Y M, PAN Z J, MA Y Y. Increased entrances to side compartments indicate incubation of craving in morphine-induced rat and tree shrew CPP models[J]. Pharmacology Biochemistry and Behavior, 2017, 159: 62. DOI: 10.1016/j.pbb.2017.07.007.
|
| [24] |
陈柳. α-突触核蛋白A53T过表达诱导帕金森病树鼩模型的研究[D]. 北京: 北京协和医学院, 2023.
|
| [25] |
KURLAN R, KIM M H, GASH D M. Oral levodopa dose-response study in MPTP-induced hemiparkinsonian monkeys: assessment with a new rating scale for monkey parkinsonism[J]. Movement Disorders, 1991, 6(2): 111. DOI: 10.1002/mds.870060205.
|
| [26] |
中华医学会神经病学分会帕金森病及运动障碍学组, 中国医师协会神经内科医师分会帕金森病及运动障碍专业. 中国帕金森病的诊断标准(2016版)[J]. 中华神经科杂志, 2016, 49(4): 268. DOI: 10.3760/cma.j.issn.1006- 7876.2016.04.002.
|
| [27] |
刘永文, 焦敏, 王岩, 等. 1990—2019年中国帕金森病疾病负担分析[J]. 疾病监测, 2024, 39(3): 363. DOI: 10.3784/jbjc.202305190234.
|
| [28] |
陈芝君, 马建, 唐娜, 等. 中国帕金森病疾病负担变化趋势分析及预测[J]. 中国慢性病预防与控制, 2022, 30(9): 649. DOI: 10.16386/j.cjpccd.issn.1004-6194.2022.09.003.
|
| [29] |
彭燕章, 叶智彰, 邹如金, 等. 树鼩生物学[M]. 昆明: 云南科技出版社, 1991.
|
| [30] |
王文广, 匡德宣, 仝品芬, 等. 树鼩的标准化研究与应用进展[J]. 实验动物科学, 2020, 37(1): 74. DOI: 10.3969/j.issn.1006-6179.2020.01.016.
|
| [31] |
奎秀莹, 王文广, 金亮子, 等. 树鼩永生化皮肤成纤维细胞株的建立及鉴定[J]. 云南农业大学学报(自然科学), 2022, 37(3): 478 DOI: 10.12101/j.issn.1004-390X(n).20 2108034.
|
| [32] |
倪荣军. 树鼩脑解剖图谱和应激相关脑环路的研究[D]. 合肥: 中国科学技术大学, 2014.
|
| [33] |
NAGATSU T, NAGATSU I. Tyrosine hydroxylase (TH), its cofactor tetrahydrobiopterin (BH4), other catecholamine-related enzymes, and their human genes in relation to the drug and gene therapies of Parkinson’s disease (PD): historical overview and future prospects[J]. Journal of Neural Transmission, 2016, 123(11): 1255. DOI: 10.1007/ s00702-018-1903-3.
|
| [34] |
BUENO-CARRASCO M T, CUELLAR J, FLYDAL M I, et al. Structural mechanism for tyrosine hydroxylase inhibition by dopamine and reactivation by Ser40 phosphorylation[J]. Nature Communications, 2022, 13(1): 74. DOI: 10.1038/s41467-021-27657-y.
|
| [35] |
YANG W W, HAO W W, MENG Z, et al. Molecular regulatory mechanism and toxicology of neurodegenerative processes in MPTP/probenecid-induced progressive Parkinson’s disease mice model revealed by transcriptome[J]. Molecular Neurobiology, 2021, 58(2): 603. DOI: 10.1007/s12035-020-02128-5.
|
| [36] |
刘长亮, 毛晓波. 致病性α-synuclein在帕金森病中的神经毒性与传播机制[J]. 广西医科大学学报, 2020, 37(2): 159. DOI: 10.16190/j.cnki.45-1211/r.2020.02.001.
|
| [37] |
李波, 角建林, 张传意, 等. MPTP诱导树鼩帕金森病模型的脑电图描记与分析[J]. 昆明医科大学学报, 2021, 42(5): 29. DOI: 10.12259/j.issn.2095-610X.S20210506.
|
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