Transcriptome Analysis of <i>Lilium</i> Siberian Induced by Melatonin and <i>LoERF96</i> Gene Cloning

LIU Ye; SUN Liang; ZHAO Guolin; PENG Mengqun; JIA Wenjie; LI Xiang; ZHANG Xiaoping; WANG Youguo

doi:10.12101/j.issn.1004-390X(n).202401006

JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science） > 2024 > 39(4): 119-130. > DOI: 10.12101/j.issn.1004-390X(n).202401006

LIU Ye, SUN Liang, ZHAO Guolin, et al. Transcriptome Analysis of Lilium Siberian Induced by Melatonin and LoERF96 Gene Cloning[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2024, 39(4): 119-130. DOI: 10.12101/j.issn.1004-390X(n).202401006

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Transcriptome Analysis of Lilium Siberian Induced by Melatonin and LoERF96 Gene Cloning

1.
College of Horticulture and Landscape, Yunnan Agricultural University, Kunming 650201, China
2.
School of Agriculture, Jinhua University of Vocational Technology, Jinhua 321017, China
3.
Flower Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, China

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Received Date: January 05, 2024
Revised Date: June 29, 2024
Accepted Date: August 21, 2024
Available Online: September 03, 2024
Published Date: September 08, 2024

Graphical Abstract

Abstract

Abstract

Purpose
To study the molecular regulatory mechanism of the germination stage of Lilium Siberian, and to screen the potential ethylene response factor (ERF) that responds to melatonin to promote the germination of Lilium Siberian.
Methods
Transcriptome sequencing analysis was performed on the samples of Lilium Siberian bulblets under melatonin treatment during the critical period, and the differentially expressed genes were functionally annotated, screening the potential ERF genes that respond to melatonin to promote the germination of lily bulblets. The screened key gene LoERF96 was cloned and bioinformatically analyzed, and the expression of LoERF96 gene was quantified by RT-qPCR in four different growth and development periods of small bulbs.
Results
According to the transcriptome data, 780 differential expressed genes were obtained, including 392 up-regulated genes and 388 down-regulated genes. The results of GO enrichment analysis showed that: the biological processes of enriched differential genes included metabolic processes, cellular processes, biological regulation, stimulus response, signaling, reproductive process, and so on. KEGG metabolic pathway analysis showed that: the metabolic pathways of the differential genes included plant hormone signal transduction, plant MAPK signaling pathway, phenylpropanoid biosynthesis, starch and sucrose metabolism, photosynthesis, carbon metabolism, protein processing in endoplasmic reticulum, and so on. The LoERF96 gene was obtained by homologous cloning, the full-length sequence of the gene was 435 bp, encoding 144 amino acids, with one AP2 conserved structural domain, the relative molecular weight of the protein was about 16.32 ku, and the theoretical isoelectric point was 4.94; it was a hydrophilic protein with no transmembrane structure, and the subcellular localization was predicted to be in nucleus. RT-qPCR results showed that: in the four different growth and development periods of lily, the relative expression level of LoERF96 gene showed a general trend of first increase and then decrease.
Conclusion
The expression of ethylene response factor LoERF96 in Lilium Siberian is regulated by melatonin, which provides a theoretical basis and reference for exploring the biological functions of the lily AP2/ERF transcription factor during the germination phase.
- lily (Lilium spp.),
- LoERF96,
- gene cloning,
- transcriptome,
- bioinformatics analysis

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References

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Transcriptome Analysis of Lilium Siberian Induced by Melatonin and LoERF96 Gene Cloning

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