Purpose To explore the effects of different mediums and temperatures on the embryonic development, morphological characteristics and motor function of larval Pelodiscus sinensis, and to optimize incubation environment parameters, providing theoretical basis and technical reference for artificial breeding and germplasm resources protection.
Methods Three groups of temperatures (28, 30, 32 ℃) and three medium environments (vermiculite, sand and no medium) were set up by orthogonal test, and the effects of temperatures and mediums on incubation rate, incubation period, larval weight, carapace length, carapace width, carapace height, and swimming speed of P. sinensis were analyzed by two-way ANOVA.
Results As the temperature increased, the incubation rate significantly increased (P<0.05), the incubation period significantly shortened (P<0.05), and the larval weight, carapace length, and swimming speed all significantly increased (P<0.05). The incubation medium had significant effects on incubation rate, larval weight, carapace length, carapace width, carapace height and swimming speed (P<0.05); among them, the above indexes of the vermiculite group were significantly higher than other groups (P<0.05). The interaction between temperature and medium had significant effects on the larval weight and swimming speed of P. sinensis (P<0.05).
Conclusion When incubating P. sinensis, the temperature sets at 32 ℃, and vermiculite is used as the incubation medium, the incubation rate is the highest, the incubation period is the shortest, and the incubating individual is relatively larger and shows stronger motor function.
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