Purpose To study the effects of adding microalgae and cow manure organic fertilizer on tobacco growth, yield and quality, and soil bacterial community.
Methods Four field experiments were conducted on microalgae, cow manure organic fertilizer, microalgae+cow manure organic fertilizer, and blank control (CK). Agronomic traits, yield, and chemical composition of cured tobacco leaves were monitored, and soil chemical properties were measured. Molecular biology techniques such as fluorescence quantitative PCR and Illumina Miseq sequencing were used to analyze the abundance and composition of soil bacterial communities.
Results Compared with CK, the application of microalgae+cow manure organic fertilizer significantly increased the content of soil nitrate nitrogen, dissolved organic carbon, available phosphorus, and available potassium, and improved soil pH; the height and number of leaves of tobacco plants during the round top stage, the contents of total nitrogen, potassium, nicotine, total sugar, and reducing sugar content, nitrogen-nicotine ratio and potassium-chlorine ratio of tobacco leaves significantly increased; the yield and output value of tobacco increased by 13.70% and 16.78% respectively; the diversity of soil bacterial communities had been improved, and the structure of soil bacterial communities had been optimized, among them, the relative abundance of Bacillus had increased, and the ability of soil bacterial communities to inhibit bacterial wilt disease had been enhanced. The typical correspondence analysis results showed that: soil pH, nitrate nitrogen, and available potassium content were key factors affecting the composition of soil bacterial community structure.
Conclusion The combination of microalgae and cow manure organic fertilizer can increase soil nutrient content, activate soil beneficial microorganisms, enhance the ability of bacterial communities to inhibit bacterial wilt, improve soil microecological environment, promote tobacco growth, and increase tobacco yield and quality.
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