Effects and Mechanism of <i>Cuscuta chinensis</i> Polysaccharides on Improving Motor Ability in Rats Based on AMPK/PGC-1α Signaling Pathway

PANG Yiting; MA Fei

doi:10.12101/j.issn.1004-390X(n).202306031

JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science） > 2024 > 39(2): 53-59. > DOI: 10.12101/j.issn.1004-390X(n).202306031

PANG Yiting, MA Fei. Effects and Mechanism of Cuscuta chinensis Polysaccharides on Improving Motor Ability in Rats Based on AMPK/PGC-1α Signaling Pathway[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2024, 39(2): 53-59. DOI: 10.12101/j.issn.1004-390X(n).202306031

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Effects and Mechanism of Cuscuta chinensis Polysaccharides on Improving Motor Ability in Rats Based on AMPK/PGC-1α Signaling Pathway

PANG Yiting^1,,
MA Fei^2, ,

1.
Sports Department of Guilin University of Electronic Science and Technology, Guilin 541004, China
2.
Sports Department of Tianjin University, Tianjin 300350, China

More Information

Received Date: June 20, 2023
Revised Date: March 06, 2024
Accepted Date: March 10, 2024
Available Online: April 10, 2024
Published Date: April 24, 2024

Graphical Abstract

Abstract

Abstract

Purpose
To study the effects and potential mechanism of Cuscuta chinensis polysaccharides (CCPs) on improving motor ability in rats, based on adenosine monophosphate activated protein kinase (AMPK)/peroxisome proliferator activator receptor γ coactivator 1α (PGC-1α) signaling pathway.
Methods
Fifty rats were divided into normal control (NC) group, exercise model (EM) group and CCPs low-dose (CCPs-L), medium-dose (CCPs-M) and high-dose (CCPs-H) groups (10, 200 and 400 mg/kg). Except NC group, all rats in the other groups needed swimming training. After swimming to exhaustion, the anti-fatigue indexes in serum, the indexes of energy metabolism and oxidative stress in liver tissue, the mRNA expression levels of AMPKα and PGC-1α, and the protein expression levels of phosphorylated AMPKα (p-AMPKα), AMPKα and PGC-1α were measured.
Results
Compared with EM group, swimming exhaustion time of CCPs groups was significantly or extremely significantly prolonged; the contents of blood urea nitrogen, lactic acid and corticosterone in serum and malondialdehyde in liver tissue were extremely significantly decreased; the contents of serum testosterone and liver glycogen, and the activities of Na⁺-K⁺-ATPase, Ca²⁺-Mg²⁺-ATPase, superoxide dismutase and glutathion peroxidase in liver tissue were significantly increased; the mRNA and protein levels of AMPKα in liver tissue had no significant changes, but the mRNA level of PGC-1α and protein levels of p-AMPKα and PGC-1α were extremely significantly increased.
Conclusion
CCPs can improve the exercise ability of rats trained with high intensity, which is related to anti-fatigue, increasing energy reserves, inhibiting oxidative stress and activating AMPK/PGC-1α signaling pathway.
- Cuscuta chinensis polysaccharides,
- exercise ability,
- anti-fatigue,
- oxidative stress,
- AMPK/PGC-1α signaling pathway

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References

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Effects and Mechanism of Cuscuta chinensis Polysaccharides on Improving Motor Ability in Rats Based on AMPK/PGC-1α Signaling Pathway

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