Purpose To identify four wheat WAKs/WAKLs genes and analyze their expression patterns responding to Puccinia striiformis f.sp. tritici (Pst) infection.
Methods Four WAKs/WAKLs genes were screened from transcriptome differentially expressed genes of wheat, which inoculated Pst in resistant variety Yun337 and susceptible variety Yun402. The gene structure, physicochemical property of protein, conserved domains and phylogenetic relationship were analyzed, and the expression patterns of four genes in wheat inoculated Pst were verified by RT-qPCR.
Results The full lengths of TraesCS1B02G004100, TraesCS6B02G459100, TraesCSU02G079300, and TraesCS3A02G122300 were 3194, 5117, 3208, and 2934 bp, respectively; they encoded 714, 960, 737 and 652 amino acids, respectively; they all included extracellular GUB domain and intracellular Ser/Thr kinase domain; and they were all located on the cell membrane. Phylogenetic analysis showed that TraesCS6B02G459100 with Os05g04460, TraesCSU02G079300 with Os02g02120, TraesCS3A02G122300 with AtWAKL15, TraesCS1B02G004100 with HORVU.MOREX.r3.5HG0503880, had a close relationship, respectively. The kinase site amino acid analysis showed that TraesCS1B02G004100 and TraesCSU02G079300 were non-RD kinases related to plant innate immune mechanism, while TraesCS6B02G459100 and TraesCS3A02G122300 were RD kinases. Gene expression analysis showed that four WAKs/WAKLs genes in wheat were expressed differently upon infection of Pst, and TraesCS1B02G004100 was down-regulated in resistant variety but scarcely expressed in susceptible variety.
Conclusion TraesCS3A02G122300 and TraesCSU02G079300 are expressed differently in both compatible and incompatible interactions between wheat and Pst; TraesCS6B02G459100 and TraesCS1B02G004100 are only respectively expressed in incompatible interaction. The expression of non-RD receptor kinase gene TraesCS1B02G004100 is inhibited, and its function and mechanism in disease resistance deserve further investigation.
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