Purpose To obtain antennal transcriptome data of Chorosoma macilentum and identify the olfactory-related genes, providing theoretical foundation for the molecular function of antennal information communication.
Methods The transcriptomic database of C. macilentum was established using Illumina HiSeq high-throughput sequencing, and the related data were annotated and expressed differently.
Results Totally 93 395 unigenes were obtained through assembling after transcriptome sequencing, of which 22 904 unigenes were annotated based on the NR database, and most of unigenes were optimally hit to genes of Halyomorpha halys (42.84%). Annotated results of GO database showed that 26 769 unigenes divided into 53 subcategories and three types (cellular components, molecular functions, and biological processes). KEGG metabolic pathway analysis showed that 10 831 unigenes were annotated into five metabolism pathways, and most unigenes were involved in signal transduction (11.62%). Based on the annotated information of unigenes, 94 genes encoding olfaction-related proteins of C. macilentum, including 54 olfactory receptor protein genes, 25 odorant binding protein genes, seven chemosensory protein genes, five gustatory receptor genes, and one sensory neuron membrane protein genes. Compared with the transcriptome of female antenna, the transcriptome of male antenna included 131 up-regulated genes and 171 down-regulated genes, and the down-regulated genes contained one chemoreceptor protein gene, and five olfactory receptor genes were expressed only in the antennae of male adults.
Conclusion In this study, the antennal transcriptomes of adult bugs are determined, and the olfactory-related genes are screened out. The results indicate that female and male adults have different recognition of external odor compounds, and the olfactory differentially expressed genes may play an important role in the information recognition processes.
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