Purpose To study the effects of Forsythia suspensa leaves extract (FSLE) on the metabolism of pregnant mare serum gonadotropins (PMSG) in liver, improving the damage to the animal organism caused by the slow metabolism of PMSG in livestock production.
Methods The active ingredient of forsythia leaves was extracted by semi-bionic enzyme alcohol method, and then thedifferent contents of FSLE were given to female Kunming rats for in vivo animals experiment. The serum of mice was collected and enzyme-linked immunosorbent assay was performed to detect the mass concentration of PMSG in the serum of each group of mice. Liver tissues of mice were collected and the kit measured the levels of oxidative stress indicators, including glutathione, superoxide dismutase, malondialdehyde and hydrogen peroxide. Western-blot was used to detect the expression levels of pregnane X receptor (PXR) and cytochrome P450 17A1 (CYP17A1) proteins in mouse liver tissues.
Results FSLE could significantly reduce the mass concentration of PMSG in the serum of mice, and it also might slow down the oxidative stress induced by PMSG metabolism. The results of Western-blot showed that FSLE could significantly down-regulate the expression levels of PXR and CYP17A1 in liver tissue.
Conclusion FSLE may attenuate oxidative stress induced by PMSG metabolism through down-regulating the expression of PXR and CYP17A1 in liver tissue.
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