Purpose To explore the sequence characteristics, functional differences and phylogenetic relationships of THRSP gene in Bovidae species.
Methods The sequences of THRSP gene and its encoded product in common species of Bovidae and in some species of non-Bovidae were downloaded from NCBI and Ensembl databases, and the obtained sequences were further compared and analyzed by the methods of comparative genomics and bioinformatics.
Results The structure of transcription region of THRSP gene was similar, with high consistency (93.2%-96.9%) of coding sequence, but the length of untranslated region and intron were different greatly. The THRSP proteins of Bovidae were all hydrophilic nucleoproteins without transmembrane region and signal peptide, and they all contained a Spot_14 or a Spot_14 superfamily conserved domain. The biological pathway of THRSP protein involved was mainly related to lipid biosynthesis, and the molecular function of this protein was mainly related to fatty acid synthase activity and protein homodimerization activity. Proteins interacted with THRSP of Bovidae were mainly involved in biological processes such as fatty acid synthesis and β-oxidation, protein metabolism and transport, material transport across cell membranes or organelles, lysosome and phagosome fusion. The amino acid composition, physicochemical properties, motif composition pattern, conserved domain, functional modification site and advanced structures of THRSP protein were highly similar among the species of Bovidae, but displayed inter-generic differences. Phylogenetic analysis showed that THRSP proteins were more closely related among species of Bovidae.
Conclusion The sequences and structures of THRSP are highly consistent in Bovidae, and its function is also similar. THRSP may play a functional role as a hydrophilic protein in the nucleus and may be involved in the regulation of lipid synthesis in Bovidae.
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