Effects of Saccharomyces cerevisiae on Growth Performance and Intestinal Bacterium of Gryllus bimaculatus

Purpose To explore the effects of Saccharomyces cerevisiae on the growth performance and intestinal bacterium of Gryllus bimaculatus De Geer.

Method Compared the growth and development indicators of G. bimaculatus fed on basic feed and feed supplemented with S. cescerevisiae, and the optimal addition group was selected. The optimal group and the control group were respectively analyzed for intestinal bacterium, and the distribution characteristics of the flora between the two groups were compared to find out the difference.

Results 1) Adding different densities of S. cerevisiae in the diet could improve the feeding amount and average weight of adult crickets, and the effect was most obvious when adding concentration of 5×10⁴ CFU/g. 2) In the group feeding on the diet supplemented with probiotic, Bacteroidota in the intestinal tract of crickets turned into a high abundance of bacteria, which might be attributed to the nutrition from S. cerevisiae. 3) The functional prediction of PICRUSt2 indicated that the relative abundance of bacterial communities related to amino acid metabolism and nucleotide metabolism in the gut of the treatment group was significantly higher than that of the control group, but the relative abundance of bacterial communities related to signal transduction and membrane transport was lower.

Conclusion After eating the feed supplemented with S. cerevisiae, the growth index of the average body weight and feeding amount of G. bimaculatus can be improved to a certain extent, and the alpha diversity and metabolic capacity of bacterial community in the intestinal tract of the crickets are also increased.