Inhibitory Effects and Mechanism Analysis of Polyphenols from Tobacco Flower on α-Amylase and α-Glucosidase

SUN Zhenchun; JIANG Tianli; GE Yonghui; YANG Hui; SU Xiankun

doi:10.12101/j.issn.1004-390X(n).202211040

JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science） > 2023 > 38(5): 808-815. > DOI: 10.12101/j.issn.1004-390X(n).202211040

SUN Zhenchun, JIANG Tianli, GE Yonghui, et al. Inhibitory Effects and Mechanism Analysis of Polyphenols from Tobacco Flower on α-Amylase and α-Glucosidase[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2023, 38(5): 808-815. DOI: 10.12101/j.issn.1004-390X(n).202211040

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Inhibitory Effects and Mechanism Analysis of Polyphenols from Tobacco Flower on α-Amylase and α-Glucosidase

1.
Guizhou Tobacco Science Research Institute, Guiyang 550081, China
2.
Food and Pharmaceutical Engineering Institute, Guiyang College, Guiyang 550005, China

More Information

Received Date: November 21, 2022
Revised Date: September 09, 2023
Accepted Date: September 10, 2023
Available Online: September 12, 2023
Published Date: October 08, 2023

Graphical Abstract

Abstract

Abstract

Purpose
To study the inhibitory effects and mechanism of polyphenols from tobacco flower on α-amylase and α-glucosidase.
Methods
The hypoglycemic activity and mechanism of polyphenols from to bacco flower in vitro were studied by enzyme inhibition, enzyme kinetics, fluorescence spectroscopy and molecular docking experiments.
Results
The 50% inhibiting mass concentration (IC₅₀) of polyphenols on α-amylase and α-glucosidase were 0.1885 mg/mL and 0.0025 mg/mL, respectively; the inhibition type was mixed type, and the static quenching of polyphenols and the enzyme protein occurred. The molecular bonding showed that the inhibition activity between small molecules and enzyme protein was formed through hydrophobic and hydrogen bonding.
Conclusion
Polyphenols from tobacco flower have significant hypoglycemic effect in vitro and have the potential to develop natural enzyme inhibitors.
- tobacco flower,
- polyphenols,
- α-amylase,
- α-glucosidase

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References (30)

References

[1]	KHAN R M M, CHUA Z J Y, TAN J C, et al. From pre-diabetes to diabetes: diagnosis, treatments and translational research[J]. Medicina, 2019, 55(9): 546. DOI: 10.3390/medicina55090546.
[2]	MATHIS D, VENCE L, BENOIST C. Beta-cell death during progression to diabetes[J]. Nature, 2001, 414(6865): 792. DOI: 10.1038/414792a.
[3]	SUN L J, SONG Y, CHEN Y J, et al. The galloyl moiety enhances the inhibitory activity of catechins and theaflavins against α-glucosidase by increasing the polyphenol-enzyme binding interactions[J]. Food & Function, 2021, 12(1): 215. DOI: 10.1039/D0FO02689A.
[4]	XU Y, WANG T S, YANG Z R, et al. Sulphonylureas monotherapy and risk of hospitalization for heart failure in patients with type 2 diabetes mellitus: a population-based cohort study in China[J]. Pharmacoepidemiology and Drug Safety, 2020, 29(6): 635. DOI: 10.1002/pds.5024.
[5]	BERGMARK B A, BHATT D L, MCGUIRE D K, et al. Metformin use and clinical outcomes among patients with diabetes mellitus with or without heart failure or kidney dysfunction[J]. Circulation, 2019, 140(12): 1004. DOI: 10.1161/CIRCULATIONAHA.119.040144.
[6]	田友清, 丁平, 张云庆. 烟草药用研究概述[J]. 中国药业, 2015, 24(9): 126.
[7]	ZOU X D, AMRIT B K, RAUF A, et al. Screening of polyphenols in tobacco (Nicotiana tabacum) and determination of their antioxidant activity in different tobacco varieties[J]. ACS Omega, 2021, 6(39): 25361. DOI: 10.1021/acsomega.1c03275.
[8]	欧阳璐斯, 赖燕华, 王予, 等. 高效液相色谱—分段检测法同时测定烟草中14种多酚类化合物[J]. 分析测试学报, 2021, 40(3): 411. DOI: 10.3969/j.issn.1004-4957.2021.03.018.
[9]	张献忠, 钟烈洲, 黄海智, 等. 大孔树脂纯化废次烟叶中烟草多酚的工艺[J]. 化工进展, 2012, 31(12): 2626. DOI: 10.16085/j.issn.1000-6613.2012.12.039.
[10]	李应金, 吴玉萍. 烟草中多酚总含量的检测[J]. 光谱实验室, 2006, 23(5): 1127. DOI: 10.3969/j.issn.1004-8138.2006.05.062.
[11]	GB/T 21733—2008. 茶饮料[S].
[12]	WANG X M, CHANG X Y, LUO X M, et al. An integrated approach to characterize intestinal metabolites of four phenylethanoid glycosides and intestinal microbe-mediated antioxidant activity evaluation in vitro using UHPLC-Q-exactive high-resolution mass spectrometry and a 1,1-diphenyl-2-picrylhydrazyl-based assay[J]. Frontiers in Pharmacology, 2019, 7(10): 826. DOI: 10.3389/fphar.2019.00826.
[13]	CHEN Y H, ZHANG R P, SONG Y M, et al. RRLC-MS/MS-based metabonomics combined with in-depth analysis of metabolic correlation network: finding potential biomarkers for breast cancer[J]. Analyst, 2009, 134(10): 2003. DOI: 10.1039/b907243h.
[14]	王闪. 酶法合成绿原酸酰化衍生物及其抗氧化和α-淀粉酶/葡萄糖苷酶抑制活性研究[D]. 无锡: 江南大学, 2021.
[15]	王晓婷. 蛹虫草多糖分子结构与α-葡萄糖苷酶抑制活性研究[D]. 天津: 天津科技大学, 2017.
[16]	潘玥. 蓝莓叶多酚提取物对α-淀粉酶和α-葡萄糖苷酶的抑制作用及其在低GI米糕中应用研究[D]. 上海: 上海海洋大学, 2021.
[17]	CORNISH-BOWDEN A. A simple graphical method for determining the inhibition constants of mixed, uncompetitive and non-competitive inhibitors[J]. Biochemical Journal, 1974, 137(1): 143. DOI: 10.1042/bj1370143.
[18]	宋菲, 陈开健, 唐敏敏, 等. 槟榔多酚提取物对α-葡萄糖苷酶活性的抑制动力学研究[J]. 热带作物学报, 2021, 42(5): 1455. DOI: 10.3969/j.issn.1000-2561.2021.05.035.
[19]	LI S Y, CHANG C Q, MA F Y, et al. Modulating effects of chlorogenic acid on lipids and glucose metabolism and expression of hepatic peroxisome proliferator-activated receptor-alpha in golden hamsters fed on high fat diet[J]. Biomedical and Environmental Sciences, 2009, 22(2): 122. DOI: 10.1016/S0895-3988(09)60034-9.
[20]	ARION W J, CANFIELD W K, RAMOS F C, et al. Chlorogenic acid and hydroxynitrobenzaldehyde: new inhibitors of hepatic glucose 6-phosphatase[J]. Archives of Biochemistry and Biophysics, 1997, 339(2): 315. DOI: 10.1006/abbi.1996.9874.
[21]	JORGE A P, HORST H, DE SOUSA E, et al. Insulinomimetic effects of kaempferitrin on glycaemia and on ¹⁴C-glucose uptake in rat soleus muscle[J]. Chemico-Biological Interactions, 2004, 149(2/3): 89. DOI: 10.1016/j.cbi.2004.07.001.
[22]	罗成. 山奈酚对胰岛素抵抗的防治作用及其机制研究[D]. 重庆: 重庆医科大学, 2015.
[23]	AHMAD G. Mechanisms of antidiabetic effects of flavonoid rutin[J]. Biomedicine & Pharmacotherapy, 2017, 96: 305. DOI: 10.1016/j.biopha.2017.10.001.
[24]	李阳, 苏艳瑜, 李国豪, 等. 槲皮素通过Nrf2通路对糖尿病大鼠胰腺氧化损伤的拮抗作用机制[J]. 食品科学, 2021, 42(5): 208. DOI: 10.7506/spkx1002-6630-20200223-260.
[25]	LO PIPARO E, SCHEIB H, FREI N, et al. Flavonoids for controlling starch digestion: structural requirements for inhibiting human α-amylase[J]. Journal of Medicinal Chemistry, 2008, 51(12): 3555. DOI: 10.1021/jm800115x.
[26]	TADERA K, MINAMI Y, TAKAMATSU K, et al. Inhibition of α-glucosidase and α-amylase by flavonoids[J]. Journal of Nutritional Science and Vitaminology, 2006, 52(2): 149. DOI: 10.3177/jnsv.52.149.
[27]	王斯慧, 黄琬凌, 陈庆松, 等. 芦丁、槲皮素对α-葡萄糖苷酶活性抑制研究[J]. 中国酿造, 2012, 31(1): 133. DOI: 10.3969/j.issn.0254-5071.2012.01.040.
[28]	王斯慧, 黄琬凌, 陈庆松, 等. 芦丁、槲皮素对α-淀粉酶抑制活性研究[J]. 食品与发酵科技, 2012, 48(3): 34. DOI: 10.3969/j.issn.1674-506X.2012.03-009.
[29]	SUN L J, MIAO M. Dietary polyphenols modulate starch digestion and glycaemic level: a review[J]. Critical Reviews in Food Science and Nutrition, 2020, 60(4): 541. DOI: 10.1080/10408398.2018.1544883.
[30]	ASEKUNOWO A K, ASHAFA A O T, OKOH O, et al. Polyphenolic constituents, antioxidant and hypoglycaemic potential of leaf extracts of Acalypha godseffiana from Eastern Nigeria: in vitro study[J]. Journal of Medicinal Plants for Economic Development, 2019, 3(1): a36. DOI: 10.4102/jomped.v3i1.36.

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Inhibitory Effects and Mechanism Analysis of Polyphenols from Tobacco Flower on α-Amylase and α-Glucosidase

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