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SUN Zhenchun, JIANG Tianli, GE Yonghui, et al. Inhibitory Effects and Mechanism Analysis of Polyphenols from Tobacco Flower on α-Amylase and α-Glucosidase[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2023, 38(5): 808-815. DOI: 10.12101/j.issn.1004-390X(n).202211040
Citation: SUN Zhenchun, JIANG Tianli, GE Yonghui, et al. Inhibitory Effects and Mechanism Analysis of Polyphenols from Tobacco Flower on α-Amylase and α-Glucosidase[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2023, 38(5): 808-815. DOI: 10.12101/j.issn.1004-390X(n).202211040
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Inhibitory Effects and Mechanism Analysis of Polyphenols from Tobacco Flower on α-Amylase and α-Glucosidase

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    • Abstract
  • Purpose To study the inhibitory effects and mechanism of polyphenols from tobacco flower on α-amylase and α-glucosidase.
    Methods The hypoglycemic activity and mechanism of polyphenols from to bacco flower in vitro were studied by enzyme inhibition, enzyme kinetics, fluorescence spectroscopy and molecular docking experiments.
    Results The 50% inhibiting mass concentration (IC50) of polyphenols on α-amylase and α-glucosidase were 0.1885 mg/mL and 0.0025 mg/mL, respectively; the inhibition type was mixed type, and the static quenching of polyphenols and the enzyme protein occurred. The molecular bonding showed that the inhibition activity between small molecules and enzyme protein was formed through hydrophobic and hydrogen bonding.
    Conclusion Polyphenols from tobacco flower have significant hypoglycemic effect in vitro and have the potential to develop natural enzyme inhibitors.
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