Effects of pH on Infection Phenotypes and Extracellular Products Properties of <i>Vibrio mimicus</i>

Zhenya KANG; Xianghui GUO; Fei XIANG; Zhenyang QIN; Yi GENG; Ping OUYANG; Defang CHEN; Xiaoli HUANG

doi:10.12101/j.issn.1004-390X(n).202207002

JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science） > 2023 > 38(1): 87-94. > DOI: 10.12101/j.issn.1004-390X(n).202207002

Zhenya KANG, Xianghui GUO, Fei XIANG, et al. Effects of pH on Infection Phenotypes and Extracellular Products Properties of Vibrio mimicus[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2023, 38(1): 87-94. DOI: 10.12101/j.issn.1004-390X(n).202207002

Citation:

PDF (1047 KB)

Effects of pH on Infection Phenotypes and Extracellular Products Properties of Vibrio mimicus

1.
College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
2.
College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China

More Information

Received Date: July 01, 2022
Revised Date: February 19, 2023
Accepted Date: February 20, 2023
Available Online: February 27, 2023
Published Date: January 29, 2023

Graphical Abstract

Abstract

Abstract

PurposeTo study the effect of pH on the phenotypes and extracellular products properties of Vibrio mimicus infection.
MethodsThe proliferation, swimming motility, self-aggregation, biofilm formation, and the protein mass concentration, enzyme activity, hemolytic, cytotoxicity and virulence of extracellular products (ECPs) of V. mimicus were determined under different pH (6.0, 7.0, 8.0, 8.5, 9.0) conditions.
ResultsAt pH 6.0-9.0, with the increase of pH value, the proliferation of V. mimicus increased first and then decreased with the strongest at pH 8.5; self-aggregation, biofilm formation and virulence of ECPs weakened first and then increased with the weakest power at pH 8.5 (P<0.05); swimming motility and protein mass concentration and cytotoxicity of ECPs showed a weakening trend, which pH 6.0 was significantly stronger than other pH value (P<0.05); in addition to the ECPs enzyme activities and hemolysis were fluctuated, which the strongest enzyme activity was at the pH 6.0 and the weakest enzyme activity at the pH 8.0; hemolytic was the strongest at pH 8.0 and the weakest at pH 8.5. Transcriptome analysis showed that: by comparing pH 7.0 and 8.5, the expression of genes related to ATP synthesis such as glycolysis pathway, TCA cycle, and oxidative phosphorylation was up-regulated in pH 8.5, while the expression of genes related to ADP synthesis such as purine synthesis and ribosomal subunit synthesis was down-regulated.
ConclusionpH has obvious influence on the V. mimicus infection-related phenotypes and ECPs characteristics, which may affect these related biological characteristics by regulating energy metabolism and membrane transport.
- pH,
- Vibrio mimicus,
- biological characteristics,
- transcriptome analysis

FullText(HTML)

References (28)

References

[1]	陆春云, 习丙文, 叶诗尧, 等. 多个环境因素对嗜水气单胞菌感染团头鲂致病力的影响[J]. 生态学杂志, 2015, 34(7): 2025. DOI: 10.13292/j.1000-4890.20150612.004.
[2]	臧学磊. 三种环境因子胁迫下罗非鱼(GIFT Oreochromis niloticus)机体免疫力的变化及其对海豚链球菌易感性的影响[D]. 南京: 南京农业大学, 2010.
[3]	GUINEY D G. Regulation of bacterial virulence gene expression by the host environment[J]. The Journal of Clinical Investigation, 1997, 99(4): 565. DOI: 10.1172/JCI119196.
[4]	HUANG L X, LIU W J, JIANG Q L, et al. Integration of transcriptomic and proteomic approaches reveals the temperature-dependent virulence of Pseudomonas plecoglossicida[J]. Frontiers in Cellular and Infection Microbiology, 2018, 8: 207. DOI: 10.3389/fcimb.2018.00207.
[5]	胡靖, 李爱华, 胡成钰, 等. 温度和pH值对嗜水气单胞菌毒力基因表达的影响[J]. 南京理工大学学报(自然科学版), 2006, 30(3): 375. DOI: 10.3969/j.issn.1005-9830.2006.03.030.
[6]	潘吉脉, 吴良涛, 杨霞, 等. 温度、pH、盐度和碳氮源对嗜水气单胞菌GZ1株生长的影响[J]. 水产学杂志, 2019, 32(4): 63. DOI: 10.3969/j.issn.1005-3832.2019.04.010.
[7]	毛秀秀, 覃映雪, 邹文政, 等. 致病性嗜水气单胞菌生物膜的形成特性[J]. 中国水产科学, 2011, 18(2): 371. DOI: 10.3724/SP.J.1118.2011.00371.
[8]	储卫华, 陆承平. 培养条件对嗜水气单胞菌胞外蛋白酶合成与分泌的影响[J]. 南京农业大学学报, 2001, 24(3): 65. DOI: 10.3321/j.issn:1000-2030.2001.03.016.
[9]	GENG Y, LIU D, HAN S, et al. Outbreaks of vibriosis associated with Vibrio mimicus in freshwater catfish in China[J]. Aquaculture, 2014, 433: 82. DOI: 10.1016/j.aquaculture.2014.05.053.
[10]	YU Z H, GENG Y, WANG K Y, et al. Complete genome sequence of Vibrio mimicus strain SCCF01 with potential application in fish vaccine development[J]. Virulence, 2017, 8(6): 1028. DOI: 10.1080/21505594.2016.1250996.
[11]	邓梦玲, 韩帅, 耿毅, 等. 南方鲇拟态弧菌的分离、鉴定及其感染的病理损伤[J]. 中国兽医学报, 2015, 35(3): 394.
[12]	张家宝, 马轲, 刘真真, 等. 鸡源卷曲乳杆菌Y68的分离鉴定及其体外益生特性评估[J]. 动物营养学报, 2022, 34(5): 3339. DOI: 10.3969/j.issn.1006-267x.2022.05.059.
[13]	LUO G, HUANG L X, SU Y Q, et al. flrA, flrB and flrC regulate adhesion by controlling the expression of critical virulence genes in Vibrio alginolyticus[J]. Emerging Microbes & Infections, 2016, 5(8): e85. DOI: 10.1038/emi.2016.82.
[14]	李玉英. 蟹源拟态弧菌外毒素的生物学特性研究[D]. 合肥: 安徽农业大学, 2003.
[15]	DHARANEEDHARAN S, HARIKRISHANAN R, BALASUNDARAM C, et al. In vitro antioxidant and antimicrobial activity of Rubus coreanus ethanolic extract, a potent biotherapeutic component for aquaculture[J]. The Israeli Journal of Aquaculture - Bamidgeh, 2013, 65(1): 1. DOI: 10.1071/MF12245.
[16]	JIA S, LI T, ZHANG X X. Integrated metagenomic and metatranscriptomic analyses of ultraviolet disinfection effects on antibiotic resistance genes and bacterial communities during wastewater treatment[J]. Ecotoxicology, 2021, 30(8): 1610. DOI: 10.1007/s10646-020-02313-1.
[17]	EILER A, JOHANSSON M, BERTILSSON S. Environmental influences on Vibrio populations in northern temperate and boreal coastal waters (Baltic and Skagerrak Seas)[J]. Applied and Environmental Microbiology, 2006, 72(9): 6004. DOI: 10.1128/AEM.00917-06.
[18]	胡兴娟, 沈飚, 张文斌. pH值及盐度对副溶血弧菌与霍乱弧菌生长影响的研究[J]. 中华医院感染学杂志, 2011, 21(22): 4855.
[19]	牟海津, 刘志鸿. 水产动物病原菌致病性胞外产物的研究进展[J]. 中国水产科学, 2000, 7(2): 93. DOI: 10.3321/j.issn:1005-8737.2000.02.021.
[20]	LIMOLI D H, JONES C J, WOZNIAK D J. Bacterial extracellular polysaccharides in biofilm formation and function[J]. Microbiology Spectrum, 2015, 3(3): 223. DOI: 10.1128/microbiolspec.MB-0011-2014.
[21]	王全民, 马遥, 刘丽钧, 等. 霍乱弧菌生物被膜发育与环境调控[J]. 生物工程学报, 2017, 33(9): 1533. DOI: 10.13345/j.cjb.170052.
[22]	BRYERS J D. Medical biofilms[J]. Biotechnology and Bioengineering, 2008, 100(1): 1. DOI: 10.1002/bit.21838.
[23]	YIN W, WANG Y T, LIU L, et al. Biofilms: the microbial “protective clothing” in extreme environments[J]. International Journal of Molecular Sciences, 2019, 20(14): 3423. DOI: 10.3390/ijms20143423.
[24]	AXEL M, CHRISTOPH V B. ATP synthesis at physiological nucleotide concentrations[J]. Scientific Reports, 2019, 9(1): 3070. DOI: 10.1038/s41598-019-38564-0.
[25]	JAMESON K H, WILKINSON A J. Control of initiation of DNA replication in Bacillus subtilis and Escherichia coli[J]. Genes, 2017, 8(1): 22. DOI: 10.3390/genes8010022.
[26]	RAMAKRISHNAN V. Ribosome structure and the mechanism of translation[J]. Cell, 2002, 108(4): 557. DOI: 10.1016/s0092-8674(02)00619-0.
[27]	BHATTACHARYYA D, DAS J. The secY gene of V. cholerae: identification, cloning and characterization[J]. Gene, 1997, 196(1/2): 261. DOI: 10.1016/S0378-1119(97)00238-2.
[28]	XIAO M F, LAI Y, SUN J, et al. Transcriptional regulation of the outer membrane porin gene ompW reveals its physiological role during the transition from the aerobic to the anaerobic lifestyle of Escherichia coli[J]. Frontiers in Microbiology, 2016, 7: 799. DOI: 10.3389/fmicb.2016.00799.

Cited By

Cited by

Periodical cited type(1)

翟玮，孔祥青，陈钊，丁祝进，葛红星，常志强. 循环率对循环水凡纳滨对虾养殖系统水质和细菌群落组成的影响. 江苏海洋大学学报(自然科学版). 2024(04): 1-8 .

Other cited types(0)

Get Citation

PDF

XML

Article views (1351) PDF downloads (13) Cited by(1)

Turn off MathJax

Article Contents

Abstract

References

Effects of pH on Infection Phenotypes and Extracellular Products Properties of Vibrio mimicus

Abstract

References

Cited by

Periodical cited type(1)

Other cited types(0)

Catalog

Export File

Citation

Format

Content