Expression and Bioinformatics Analysis of <i>FASN</i> Gene in Mammary Tissue of Dehong Dairy Buffalo

PANG Shuanglong; XU Weiwei; XU Zhengyao; WANG Mengdi; ZHAO Hongxiao; XU Sihui; YANG Zhong; LI Weizhen

doi:10.12101/j.issn.1004-390X(n).202206059

JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science） > 2023 > 38(5): 779-785. > DOI: 10.12101/j.issn.1004-390X(n).202206059

PANG Shuanglong, XU Weiwei, XU Zhengyao, et al. Expression and Bioinformatics Analysis of FASN Gene in Mammary Tissue of Dehong Dairy Buffalo[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2023, 38(5): 779-785. DOI: 10.12101/j.issn.1004-390X(n).202206059

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Expression and Bioinformatics Analysis of FASN Gene in Mammary Tissue of Dehong Dairy Buffalo

1.
College of Veterinary Medicine, Yunnan Agricultural University, Kunming 650201, China
2.
Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China
3.
Yunnan Biological Pharmaceutical Co., Ltd., Kunming 650503, China

More Information

Received Date: June 28, 2022
Revised Date: August 07, 2023
Accepted Date: August 10, 2023
Available Online: September 21, 2023
Published Date: October 09, 2023

Graphical Abstract

Abstract

Abstract

Purpose
To study the expression level of FASN gene in mammary tissue of Dehong dairy buffalo and the biological characteristics of FASN protein in buffalo.
Methods
Milk fat percentage was detected by milk composition analyzer. High, medium and low groups (groups H, M, and L) were divided by different milk fat percentage. The expression level of FASN gene in mammary tissue was detected by qPCR. The correlation of FASN gene expression level and milk fat percentage were analyzed. Physicochemical property and structural characteristics of FASN protein of buffalo were analyzed by bioinformatics method.
Results
The expression level of FASN gene in mammary tissue of Dehong dairy buffalo in group H was extremely significantly higher than that in groups L and M (P<0.01), and group M was extremely significantly higher than that in group L (P<0.01). The expression level of FASN was extremely significant positive correlated with the milk fat percentage (P<0.01). The molecular formula of buffalo FASN protein was C₁₂₁₈₈H₁₉₃₂₇N₃₃₆₃O₃₆₃₁S₁₀₉, molecular weight was 274.56 ku. This protein was unstable, and mainly distributed in the nucleus. There were two domains in FASN, of which were all related to lipid anabolism. The secondary structure mainly consisted of α-helix and random coil. The evolutionary tree and homology analysis showed that the FASN of buffalo was closely related to the yak and domestic cattle. The FASN mainly interacted closely with ACACB, ACACA and ACLY.
Conclusion
FASN gene expression in mammary tissue can promote the increase of milk fat percentage.
- Dehong dairy buffalo,
- FASN gene,
- milk fat percentage,
- gene expression level,
- bioinformatics

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References (22)

References

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