Expression and Bioinformatics Analysis of FASN Gene in Mammary Tissue of Dehong Dairy Buffalo

Purpose To study the expression level of FASN gene in mammary tissue of Dehong dairy buffalo and the biological characteristics of FASN protein in buffalo.

Methods Milk fat percentage was detected by milk composition analyzer. High, medium and low groups (groups H, M, and L) were divided by different milk fat percentage. The expression level of FASN gene in mammary tissue was detected by qPCR. The correlation of FASN gene expression level and milk fat percentage were analyzed. Physicochemical property and structural characteristics of FASN protein of buffalo were analyzed by bioinformatics method.

Results The expression level of FASN gene in mammary tissue of Dehong dairy buffalo in group H was extremely significantly higher than that in groups L and M (P<0.01), and group M was extremely significantly higher than that in group L (P<0.01). The expression level of FASN was extremely significant positive correlated with the milk fat percentage (P<0.01). The molecular formula of buffalo FASN protein was C₁₂₁₈₈H₁₉₃₂₇N₃₃₆₃O₃₆₃₁S₁₀₉, molecular weight was 274.56 ku. This protein was unstable, and mainly distributed in the nucleus. There were two domains in FASN, of which were all related to lipid anabolism. The secondary structure mainly consisted of α-helix and random coil. The evolutionary tree and homology analysis showed that the FASN of buffalo was closely related to the yak and domestic cattle. The FASN mainly interacted closely with ACACB, ACACA and ACLY.

Conclusion FASN gene expression in mammary tissue can promote the increase of milk fat percentage.