PurposeTo lay a foundation for hybrid combination selection and molecular marker-assisted selection breeding of hulless barley (Hordeum vulgare L. var. nudum Hook. f).
MethodsGenetic diversity among 39 parental materials of hulless barley was analyzed by (simple sequence repeat, SSR) markers, and five main agronomic traits, such as plant height, spikelet density, tiller number, grain number per plant and 1000-grain weight were measured, and then the correlation between SSR markers and agronomic traits was analyzed.
Results1) The average polymorphism information content of 27 SSR markers was 0.6125, and the polymorphism information content of Bmag0770 and Scssr09398 was the highest. 2) The average genetic distance between 39 hulless barley parents was about 0.3612, and that between Peony hulless barley and 83A-19-1 was the largest, which was suitable for the selection of excellent parents. 3) Cluster analysis and population genetic structure analysis divided the materials into three subgroups, containing 19, 10 and 10 materials, respectively. 4) A total of 11 molecular markers were found to be associated with plant height, spikelet density, tiller number, grain number per plant and 1000-grain weight, etc., the explanatory rates of phenotypic variation were 10.15%-64.79%, 6.26%-29.39%, 13.70%-39.20%, 16.54%-17.67% and 10.81%-42.73%, respectively, and the 11 associated markers were detected in six linkage groups.
ConclusionThe 39 hulless barley parental materials tested in this study have little genetic differences and close genetic relationships, and the 11 molecular markers detected can be used as molecular markers to develop candidate genes associated with agronomic traits.
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