Analysis on the SRAP Diversity of Pinus armandii Clones Seed Orchard in Chuxiong

PurposeTo analyze the genetic diversity among different clones in Pinus armandii seed orchard in Chuxiong City, Yunnan Province.

MethodsGenomic DNA was extracted from the tissue needles of 96 clones from six provenances of P. armandii by improved CTAB method, and genetic diversity was analyzed by SRAP markers.

ResultsA total of 26 pairs of SRAP markers with clear bands, stable PCR reaction, and high polymorphism were genotyped. The average percentage of polymorphic loci was 81.22%, Shannon’s diversity index (I) was 0.394 4, Nei’s gene diversity index (H) was 0.2595, and genetic differentiation coefficient (G_st) among provenances was 0.116 9. The variation among six provenances was low in the seed orchard of P. armandii, while the genetic variation within provenances had high level. Six provenances were clustered into three groups, and there was no significant correlation between genetic diversity and geographical distance (P>0.05).

ConclusionThe genetic diversity among six provenances of P. armandii is low, and the variation is existed mainly in various provenances. Geographical distance has no significant effect on the genetic distance of clonal materials. The molecular markers will be used mainly, and the geographical distance will be referred in breeding of P. armandii, especially in the selection of the parents of crosses in this seed orchard.