Establishment of Somatic Embryogenesis System in Vitis vinifera L. cv. ‘Marselan’

PurposeTo establish somatic embryogenesis in Vitis vinifera L. cv. ‘Marselan’, and to provide new data for enhancing the theoretic basis for the establishment of genetic transformation system.

MethodsFlower buds and immature seeds of Marselan were used as explant, and the effects of different hormone combinations and hormone levels (Fow1-6 and Ims1-6) on the somatic embryogenesis were studied. The hormone of medium were supplemented with 2,4-dichlorophenoxyacetic acid, melatonin, N-(phenylmethyl)-9H-purin-6-amine and thidiazuron.

ResultsFlower buds from Marselan occurred the highest callus induction rate in Fow6 medium, and the immature seed from Marselan occurred the highest callus induction rate in Ims2 and Ims5 medium, with the callus induction rate from 67.19% to 78.00%. After 12 to 14 weeks of culture, the callus were transferred to the EM6 medium. Among them, the flower buds had no somatic embryos formation, but the immature seeds callus on the Ims3 medium were successfully induced somatic embryogenesis, and the induction rate was 6.77%, which could be formed normally on EM6 medium. SCM6 medium was beneficial to cell suspension cultures, and a large number of cell masses with good proliferation could be obtained. Cytological observation showed that callus cells were in good condition.

ConclusionThe somatic embryogenesis and the cell suspension cultures system of V. vinifera L. cv. ‘Marselan’ is firstly established, providing technical support for the establishment of genetic transformation system, the gene-verification and molecular breeding.