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Fanzhang WU, Xudong SUN, Huini XU. Screening and Identifying Interaction Proteins of NBS1 in Arabidopsis thaliana[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2023, 38(4): 558-565. DOI: 10.12101/j.issn.1004-390X(n).202202008
Citation: Fanzhang WU, Xudong SUN, Huini XU. Screening and Identifying Interaction Proteins of NBS1 in Arabidopsis thaliana[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2023, 38(4): 558-565. DOI: 10.12101/j.issn.1004-390X(n).202202008
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Screening and Identifying Interaction Proteins of NBS1 in Arabidopsis thaliana

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    • Abstract
  • PurposeTo screen the interaction protein and explore the new function of NBS1 protein in Arabidopsis thaliana, laying the foundation for further research.
    MethodsThe yeast two-hybrid technology was used to screen the interaction proteins of NBS1 from A. thaliana cDNA library. BLAST alignment, subcellular localization analysis and gene ontology annotations were performed on the obtained sequences.
    ResultsThere were 97 proteins interacted with NBS1 in 221 positive clones by BLAST after sequencing, including membrane proteins, transporters and folding proteins. These proteins were mainly localized in cytoplasm, nucleus and chloroplast, which were mainly enriched in four molecular functions, five cellular components and 13 biological processes.
    ConclusionThe proteins interacting with NBS1 play an important role in stimulus response, signal transduction and cell metabolism. It is also confirmed that NBS1 is a multifunctional protein, but its function and molecular mechanism need further studies.
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