Quality Analysis of Panax notoginseng New-type Decoction Pieces Based on Functional Ingredients-pharmacodynamic Evaluation

PurposeTo study the quality and biological effects of new types of fresh Panax notoginseng quick-dried powder, freeze-dried P. notoginseng powder and traditional hot air-dried powder of P. notoginseng, the differences in composition and efficacy caused by drying methods were investigated, searching the potential pharmacodynamic activity of the internal components and providing a scientific basis for the development of new types of P. notoginseng pieces.

MethodsThe contents of monomer saponins, total polysaccharides, total flavonoids, total phenols and total protein components in three dried decoction pieces were analyzed by high performance liquid chromatography (HPLC) and ultraviolet spectrophotometry. The pharmacological efficacy differences of three different dry decoction pieces were studied by establishing relevant pharmacodynamic models, and the results were analyzed by component-drug efficacy correlation.

ResultsCompared with hot air-dried powder, the contents of ginsenosides Rg₁ and Rb₁, total polysaccharides and total phenols in fresh P. notoginseng quick-dried powder were increased by 4.02%, 4.48%, 278.40% and 29.33%, respectively; the contents of Rg₁ and Rb₁, the sum of five saponins, total polysaccharide and total protein in freeze-dried P. notoginseng powder increased by 16.33%, 12.17%, 10.03%, 49.76% and 22.85%, respectively. The ingredients-pesticide effect correlation analysis showed that: the frequency of analgesic writhing was significantly negatively correlated with total polysaccharide content (P<0.05); the prothrombin time (PT) was extremely significantly positively correlated with Rg₁ content (P<0.01), and significantly positively correlated with the Rb₁ content (P<0.05); thrombin time (TT) was significantly positively correlated with ginsenoside Re content (P<0.05); analgesic latency time was extremely significantly positively correlated with Rg₁ content (P<0.01), and significantly positively correlated with total protein content (P<0.05). The pesticide effect verification test found that: total polysaccharide and total protein had anticoagulant and analgesic activities.

ConclusionThe ingredients and pesticide effect of freeze-dried P. notoginseng powder and fresh P. notoginseng quick-dried powder have been improved, and total polysaccharide and total protein have anticoagulant and analgesic effects. Rg₁, Re and Rg₁, and Rb₁ plays a leading role in analgesic activity, prolong TT, and prolong PT, respectively.