Rapid Determination of Anthracnose Resistance of Illicium verum Clones

PurposeTo establish a simple, accurate and reliable disease resistance evaluation system of Illicium verum clones resistant to anthracnose, providing a theoretical basis for the selection and application of I. verum clones.

MethodsThe detached leaf inoculation method and the seedling leaf inoculation method were used to determine the resistance level of 23 I. verum clones to anthracnose.

ResultsThe results of 22 clones of 23 I. verum clones determined by two inoculation methods were consistent, and the consistency was as high as 95.65%. The results showed that Fujin No.14 and Heiye No.1 were disease-resistant clones, nine clones such as Fujin No.4, Fujin No.5 and Fujin No.6 were moderate-resistant clones, six clones including Fujin No.2, Fujin No.3 and Fujin No.7 were moderate-susceptible clones, Fujin No.8 and Fujin No.16 were susceptible clones, Fujin No.1, Fujin No.11 and Fujin No.12 were high-susceptible clones.

ConclusionBoth the two inoculation methods can reflect the differences in disease resistance of I. verum clones. The detached leaf inoculation method has the advantages of easy operation, fast pathogenesis, no harm to plants and short time-consuming, and its disadvantage is that the results of identification may not be consistent with the actual resistance performance, and it can be used as an important assistant means for the identification of I. verum anthracnose. The identification results of the seedling inoculation method are more reliable, but the disadvantage is that the damage to the plant is too great, the leaves after inoculation are serious, the plants are difficult to recover, and appropriate environmental conditions such as temperature and humidity are required.