Effects of Earthworm Protein Extract on Intestinal Mucosal Immune Function in Immunosuppressed Mice

PurposeTo study the effects of earthworm protein extract on the intestinal mucosal immune function of immunosuppressed mice.

MethodsA total of 30 Kunming mice were randomly divided into six groups: blank control group, immunosuppressive model group (CTX), immunosuppressive+high (CTX+EEH)/medium (CTX+EEM)/low (CTX+EEL) earthworm protein dose group, and earthworm protein group (EE). All groups were gavaged intragastally for consecutive 20 days. On the 18th day, CTX, CTX+EEH, CTX+EEM and CTX+EEL groups were intraperitoneously injected with 50 mg/kg cyclophosphamide for three consecutive days. Immune organ index was calculated, intestinal cytokines and mucosal barrier proteins were detected.

ResultsThe thymus index, spleen index and intestinal cytokines in CTX group were significantly lower than those in blank control group (P<0.05). Compared with CTX group, spleen index, contents of cytokines TNF-α, IFN-γ and IL-10, content of sIgA and gene expression of ZO-1 and Occludin in CTX+EEH group were significantly increased (P<0.05). The spleen index, IL-10 content and gene expression of mucosal barrier proteins ZO-1, Claudin-1 and Occludin in CTX+EEM group were significantly higher than those in CTX group (P<0.05).

ConclusionEarthworm protein can improve the intestinal mucosal immune function and enhance the intestinal immune barrier in immunosuppressed mice. This study will provide a theoretical basis for earthworm protein in the prevention and treatment of animal diseases.