Study on the Protective Effect of PolyphyllinⅠon HaCat Cells Injured by Ultraviolet Ray

PurposeTo study the protective effects and molecular mechanism of polyphyllin Ⅰ(PPⅠ) on the HaCaT cell exposed to acute medium wave ultraviolet rays (UVB) injury, providing a theoretical basis for application of PPⅠ in daily chemical products.

MethodsMethyl thiazolyl tetrazolium method was used to detect the survival rate of HaCaT cell treated with different concentrations of PPⅠ, and the non-toxic PPⅠ concentration was screened out. HaCaT cells were randomly divided into four groups: blank control group, UVB treatment group (21.6 mJ/cm²), 0.250 μmol/L PPⅠ+UVB treatment group, and 0.500 μmol/L PPⅠ+UVB treatment group; crystal violet staining was used to explore the effects of two concentrations of PPⅠ on the proliferation of HaCaT cells after UVB treatment. HaCaT cells were analyzed by Western-blot for protein expression of poly ADP-ribose polymerase (PARP1), cleaved-PARP1, acetyl-lysine, mitochondrial recombinant superoxide dismutase 2 on the K68 loci (K68-SOD2) and sirtuin3 (SIRT3).

ResultsCompared with the blank control group, the UVB group showed a significant decrease in the survival rate of HaCaT cell, a significant increase in the expression of cleaved-PARP1 and K68-SOD2, and a significant decrease in the expression of SIRT3, modeling success. Compared with the UVB group, treatment with each dose of PPⅠ significantly increased the survival rate of HaCaT cell after UVB irradiation, increased the expression of SIRT3, and decreased the acetylation level of K68-SOD2, which in turn decreased the expression of proapoptotic protein cleaved-PARP1 and decreased the apoptosis level of HaCaT cells.

ConclusionPPⅠmay play a protective role in HaCaT cells by enhancing SIRT3 activity, deacetylating SOD2 to enhance SOD2 activity, and alleviate oxidative stress and apoptosis caused by UVB irradiation of HaCaT cells.