PurposeTo study the inhibitory effect of corn peptide combined with aerobic exercise on obesity mice induced by high-fat diet, and to explore the molecular mechanism of the effect.
MethodsFour-week-old ICR mice were randomly divided into standard control group (CON, feeding standard diet), high-fat group (DIO) and high-fat combined with aerobic exercise group (DEX) (both feeding high-fat diet), high-fat combined with corn peptide group (DCP) and the high-fat combined with corn peptide and aerobic exercise group (DCE) (both feeding the high-fat diet supplemented with corn peptide). At the same time, DEX and DCE group underwent aerobic exercise six times a week. After eight weeks of feeding, body weight, perirenal fat, epididymal fat, subcutaneous fat and liver tissue weight of mice were measured. Total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL) and high density lipoprotein (HDL) content in serum were detected. The expression levels of genes related with adipogenesis (PPARγ and C/EBPα) and lipogenesis (FAS) were determined by Western-blot.
ResultsDietary supplementation of corn peptide had a certain inhibitory effect on the body weight, liver and fatty tissue weight, lipid level and expression of lipid synthesis related genes in liver and adipose tissue of mice, but not significant (P>0.05). Corn peptide combined with aerobic exercise could reduce or significantly reduce the body weight, liver and fatty tissue weight (P>0.05 or P<0.05), significantly reduce serum TG and LDL content (P<0.05), and also significantly inhibited the expression level of PPARγ in obese mice (P<0.05).
ConclusionCorn peptide combined with aerobic exercise can inhibit the expression of adipocyte differentiation transcriptional regulator PPARγ, which has anti-obesity effect on the high-fat diet mice. This study provides a scientific basis for the development of corn peptide as an auxiliary functional food for weight loss through exercise.
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