Study on the Expression of CD36 Gene in Mammary Gland Tissue of Dehong Dairy Buffalo

PurposeTo study the expression differences of cluster of differentiation 36 (CD36) gene in mammary gland tissue of Dehong dairy buffalo with different milk fat content.

MethodsA total of 216 healthy Dehong dairy buffaloes with the same breeding community and feeding conditions were selected. Milk samples were collected and fat contents were detected with a milk component analyzer. The milk fat content (7.5±0.5)% as the benchmark, high, medium and low milk fat content group were divided (set H, M and L group, respectively), three Dehong dairy buffaloes with the same parity and similar age were selected in each group. All animals were sampled in the middle of location. After slaughter, mammary gland tissue was collected, total RNA wasextracted, and real-time fluorescence quantitative PCR was used to detect the mRNA expression level of CD36 gene, and the correlation analysis was carried out between the expression level and the milk fat content.

ResultsThe mRNA expression level of CD36 gene in group H was extremely significantly higher than that in the groups M and L (P<0.01), and that in group M was extremely significantly higher than that in group L (P<0.01). Correlation analysis indicated that the expression of CD36 gene had an extremely significant positive correlation with milk fat content (P<0.01).

ConclusionCD36 gene expression could promote milk quality of Dehong dairy buffalo.