Isolation, Sequence Molecular Characteristics and Tissue Expression of <i>MAPK10</i> Gene in Lijiang Pig

Guoxiang LAN; Siqi JIN; Dawei YAN; Shaoxiong LU; Yijia CUI; Guomei LI; Hailian LI; Xinxing DONG

doi:10.12101/j.issn.1004-390X(n).202106007

JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science） > 2022 > 37(1): 82-90. > DOI: 10.12101/j.issn.1004-390X(n).202106007

Guoxiang LAN, Siqi JIN, Dawei YAN, et al. Isolation, Sequence Molecular Characteristics and Tissue Expression of MAPK10 Gene in Lijiang Pig[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2022, 37(1): 82-90. DOI: 10.12101/j.issn.1004-390X(n).202106007

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Isolation, Sequence Molecular Characteristics and Tissue Expression of MAPK10 Gene in Lijiang Pig

1.
Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China
2.
Xuanwei Livestock and Poultry Improvement Workstation, Yunnan Province, Xuanwei 655400, China

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Received Date: June 14, 2021
Revised Date: September 01, 2021
Accepted Date: September 05, 2021
Available Online: December 03, 2021
Published Date: January 30, 2022

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Abstract

Abstract

PurposeTo isolate mitogen-activated protein kinase (MAPK10) gene from Lijiang pig and to analyse the expression of mRNA and protein in tissue.
MethodsThe MAPK10 gene in Lijiang pig was amplified by PCR, and the bioinformatics software was used for sequence analysis. The relative expression level of MAPK10 gene in nine tissues of Lijiang pig including brain, liver, heart, spleen, lung, kidney, pancreas, back fat and longissimus dorsi, which was detected by real-time fluorescence quantitative PCR. Taking the Large White pig as a control, real-time fluorescent quantitative PCR and Western-blotting were used to detect the difference in mRNA and protein expression in the back fat of Lijiang pig and Large White pig.
ResultsThe total length of MAPK10 gene sequence was 1281 bp, which encodes 426 amino acids. MAPK10 protein was a hydrophilic protein without transmembrane domain and signal peptide. There were 44 phosphorylation sites and two glycosylation sites. The secondary structure of Lijiang pig MAPK10 was mainly consisted of α-helices and random coils. The homology between the sequence of Lijiang pig MAPK10 gene and that of human, cattle, goat, sheep, mouse, dog, monkey, and chicken was 95.3%, 93.5%, 93.2%, 93.0%, 92.6%, 91.2%, 90.5% and 85.6%, respectively. MAPK10 mRNA was widely expressed in the nine tissues of Lijiang pigs with highest expression level in brain. The mRNA expression level of MAPK10 gene in back fat of Lijiang pigs was extremely significantly lower than that of the Large White pig (P<0.01), and the protein expression level was also lower than that of the Large White pigs (P>0.05).
ConclusionMAPK10 gene can be used as a candidate gene for studying fat deposition traits in Lijiang pigs, and the result can provide reference for the study of fat deposition of pigs.
- Lijiang pig,
- MAPK10 gene,
- bioinformatics analysis,
- tissue expression,
- fat deposition

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Isolation, Sequence Molecular Characteristics and Tissue Expression of MAPK10 Gene in Lijiang Pig

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