Effects of Leptin on the mRNA Expression of Lipid Droplets Formation Related Genes in Porcine Primary Adipocytes

Yongjiang HE; Hui XIAO; Ying HUANG; Minghua YANG; Sumei ZHAO; Hongbin PAN; Chen LIU; Zeqin PENG

doi:10.12101/j.issn.1004-390X(n).202105054

JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science） > 2022 > 37(1): 91-96. > DOI: 10.12101/j.issn.1004-390X(n).202105054

Yongjiang HE, Hui XIAO, Ying HUANG, et al. Effects of Leptin on the mRNA Expression of Lipid Droplets Formation Related Genes in Porcine Primary Adipocytes[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2022, 37(1): 91-96. DOI: 10.12101/j.issn.1004-390X(n).202105054

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Effects of Leptin on the mRNA Expression of Lipid Droplets Formation Related Genes in Porcine Primary Adipocytes

1.
Faculty of Animal Science and Technology, Yunnan Agricultural University, Yunnan Key Laboratory of Animal Nutrition and Feed, Kunming 650201, China
2.
Diqing Tibetan Autonomous Prefecture’s Secondary Vocational School for Nationalities, Diqing 674499, China
3.
College of Biotechnology and Engineering, West Yunnan University, Lincang 677000, China

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Received Date: May 23, 2021
Revised Date: September 05, 2021
Accepted Date: September 08, 2021
Available Online: November 30, 2021
Published Date: January 30, 2022

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Abstract

Abstract

PurposeTo study the effect of leptin (LEP) on the mRNA expression of lipid droplet related genes FITM2, PLIN1 and PLIN2 in porcine primary adipocytes.
MethodsSubcutaneous primary adipocytes from DLY pigs at 15-day was treated with different mass concentrations LEP including 0, 50, 100 and 150 ng/mL for 48 hours, and the content of triglyceride (TAG) in cells were measured, and the mRNA expression levels of lipid droplet related genes FITM2, PLIN1 and PLIN2 were detected using qPCR technology.
ResultsCompared with 0 ng/mL LEP, the content of TAG and the mRNA expression levels of FITM2 and PLIN2 were significantly increased in 100 ng/mL LEP (P<0.05), the mRNA expression level of PLIN1 was significantly down-regulated (P<0.05). TAG content of 150 ng/mL LEP was significantly lower than 50 and 100 ng/mL LEP (P<0.05), and the mRNA expression levels of PLIN1 and PLIN2 were significantly higher than 50 and 100 ng/mL LEP (P<0.05).
ConclusionLEP can promote the mRNA expression of FITM2 and PLIN2, it can promote or inhibit the mRNA expression of PLIN1, however, there are differences in the mass concentration of LEP in promoting or inhibiting the expression of lipid droplet related genes.
- porcine primary adipocyte,
- leptin (LEP),
- lipid droplets related genes

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Effects of Leptin on the mRNA Expression of Lipid Droplets Formation Related Genes in Porcine Primary Adipocytes

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