Effect of 2,4-epibrassinolide on the Nitrogen Uptake and Translocation of Grape Seedlings and Nitrogen Content in Soil

PurposeTo study the effect of 2,4-epibrassinolide (EBR) on the nitrogen uptake of grape seedlings and the nitrogen content in the soil.

MethodsUsing Summer Black grape as material, the effects of different mass concentrations (0, 0.5, 1.0, 1.5, and 2.0 mg/L) of EBR on the nitrogen uptake and translocation of grape seedlings and nitrogen content in soil were studied by pot experiment.

ResultsFoliar spraying of EBR with different mass concentrations could increase the nitrogen content of the whole plant of Summer Black grape seedlings, and increase the nitrogen uptake, nitrogen translocation, transport efficiency and transport contribution rate of the roots, stems and leaves of the grape seedlings, and the best effect was when the mass concentration of EBR was 1.5 mg/L. Spraying different mass concentrations of EBR could reduce the nitrogen translocation amount coefficient and nitrogen translocation coefficient of the Summer Black grape seedlings. Spraying different mass concentrations of EBR improved the nitrogen harvest index of roots and stems, and the effect was better when the EBR mass concentration was 1.5 mg/L; but the leaf nitrogen harvest index decreased. After spraying EBR, the content of soil available nitrogen increased, and it was the highest at 1.5 mg/L EBR treatment. Spraying EBR increased the soil nitrate nitrogen content, while the soil ammonium nitrogen content decreased.

ConclusionSpraying EBR can improve the nitrogen transport and uptake of grape seedlings, and the best effect is when the mass concentration of EBR is 1.5 mg/L. At the same time, the nitrogen form in soil can change in the direction of beneficial to the uptake and utilization of grape seedlings.