Proliferation Characteristics of Goat Pox Virus in Goat Testicular Cells of Different Ages

PurposeTo determine the proliferation efficiency of goat pox virus (GTPV) AV41 attenuated vaccine strain in primary testicular cells of goats at different ages, and to provide datas to support the selection of cultured cells for virus or vaccine strains.

MethodsTwo ages of cells (7-day-old and 60-day-old goat testicular cells) were isolated and cultured in traditional way. The growth efficiency of the two ages of cells was compared by the long-term dynamic cell imaging and data analysis system, and then GTPV was inoculated to observe the morphological characteristics. The propagation efficiency of GTPV was compared by indirect immunofluorescence (IFA) and fluorescent quantitative PCR (qPCR).

ResultsThere was no obvious differences in morphological characteristics and growth rate between the two ages of cells. After GTPV inoculation, the two ages of primary goat testicular cells could produce cytopathic effect (CPE), but there were differences in the occurrence time and CPE characteristics. IFA and qPCR results showed that the propagation efficiency of GTPV in 60-day-old goat testicular cells was higher than that in 7-day-old goat testicular cells.

ConclusionBoth 7-day-old and 60-day-old goat primary testicular cells can develop obvious CPE after inoculation with GTPV. GTPV can proliferate in both cells, but the propagation efficiency is different. These results may be useful for the culture of GTPV attenuated strain with goat testis cells.