Diversity of Endophytic Fungi from Yunyan87 and Evaluation of Growth-promoting

PurposeThe endophytic fungi community and diversity of Yunyan87, main tobacco cultivar in Yunnan Province and the growth promoting effects on different tobacco seedings were elucidated after isolation and identification.

MethodThe endophytic fungi of mature tobacco plants were separated after surface disinfection and tissue separation. The effects of the growth promotion of endophytic fungi were evaluated by the fresh weight, root fresh weight, fresh weight of the aboveground, plant height, maximum leaf length, maximum leaf width and root length of the seedlings of Yunyan87, Yunyan97, K326 and Honghuadajinyuan after addition of the fermentation broth.

ResultsA total of 53 endophytic fungi belonging to 29 genera were isolated and identified. Fusarium oxysporum, Aspergillus terreus and Talaromyces minioluteus showed significant promoting effect of tobacco growth were screened out, and the concentration of these three endophytic fungi in Yunyan87 were relatively high. Compared with the control group, F. oxysporum could increase the plant height, fresh weight and aboveground fresh weight of Yunyan97 seedling by 107.64%, 153.97% and 167.09%, respectively. While A. terreus showed the most significant effect on increasing the biomass of Yunyan87 tobacco seedlings, the fresh weight, root fresh weight and aboveground fresh weight increased by 172.85%, 209.57% and 169.18%, respectively. T. minioluteus increased the height of Yunyan87 and K326 tobacco seedlings by 71.66% and 50.55%.

ConclusionThere were abundant endophytic fungi living in Yunyan87 which had organizational differences. F. oxysporum (001E-5) and A. terreus (001H-10) increased the fresh weight of Yunyan97, Yunyan87 and K326 by over 91%. This study provided a theoretical basis for the development of tobacco growth promoting agents.