Predicting miRNA Targeting FASN, HMGCR and CYP11A1 Genes in Moschus berezovskii Based on SWChen System

PurposeTo predict the miRNAs targeting FASN, HMGCR and CYP11A1 genes in Moschus berezovskii for providing the reference about mechanism of musk biosynthesis.

MethodWe acquired chemical components of musk to seek out key enzymes by metabolomics sequencing. Then, sequences of FASN, HMGCR and CYP11A1 mRNA sequences as well as miRNA sequences data of M. berezovskii were obtained by transcriptomic sequencing of musk gland. SWChen system written by ourselves had been used to screen candidate miRNAs of pertinent genes and had been verified the veracity. Meanwhile, the predicted results of Bos taurus, Capra hircus and Ovis aries species were used to compare to get candicate miRNAs regulating genes of M. berezovskii.

ResultThe chemical substances of M. berezovskii mainly included in carbon chain fatty acid derivatives, aromatic, sterols and steroid derivative matters. The predicted results revealed that the miRNAs regulating FASN gene expression of M. berezovskii mainly constituted as miR-24-3p and miR-30b-3p. The predicted miR-29b, miR-146a, miR-181b-3p and miR-429 family preferentially had influence on HMGCR gene expression. Then, miR-532-3p and let-7 family had been displayed as the modulation to CYP11A1 gene expression. Further, the miR-205 family and miR-143 family could jointly regulate the expression of the three genes.

ConclusionThe above predicted miRNAs are candidate regulators targeting the expression of FASN, HMGCR and CYP11A1 in M. berezovskii, which had the referential value for the analysis of the biosynthesis mechanism of musk in M. berezovskii.