SSR Markers Analysis of Genetic Diversity in Pinus armandii Clones from Seed Orchard in Zixi Mountain

PurposeIn order to illuminate the genetic structure and genetic relationship of various provenance Pinus armandii clones from seed orchard of Zixi Mountain in Chuxiong City, SSR genetic diversity analysis was performed on 97 clones from six provenances of the seed orchard.

MethodThe modified CTAB method was used to extract the DNA of 97 P. armandii colones, and then 26 pairs of high-polymorphism primers screened in the previous stage were used for SSR-PCR amplification of all DNA samples, and their polymorphism band information was counted. Finally, POPGEN 1.32, NTSYS 2.10 and Structure 2.3.1 software were used to analyze their genetic diversity.

Result1) At the population level, the range of N_a in six provenances was from 3.730 8 to 4.807 7, with an average of 4.237 1, while N_e was 2.901 1, H was 0.637 6, I was 1.186 5. It showed that P. armandii of different provenance all had high genetic diversity. 2) The cluster analysis showed that the genetic differences of provenances with close geographical distance were not necessarily close, and the UPGMA cluster diagram did not classify the provenances with close geographical location into one group. 3) The average N_m value of P. armandii gene flow was 4.150 3, indicating that there was more genetic exchange among P. armandii provenances.

ConclusionThe genetic variation among provenances of P. armandii clones was not high, and the main genetic variation was within the provenance. The results of this study could provided theoretical basis and practical guidance for the genetic improvement of P. armandii in seed orchard by using different clones.