The Isolation and Identification of Lactic Acid Bacteria from Silage Corn

PurposeThe separation, screening and identification of lactic acid bacteria from silage corn provides excellent strains for silage, which has important guiding significance for improving the quality of silage.

MethodUsing the whole plant silage maize silaged for 60 days as the material, the strain was isolated and cultivated by the method of MRS plate separation and culture. Furthermore, the excellent lactic acid bacteria was screened by morphological characteristics, acid production rate, growth curve and analysis of 16S rDNA sequence.

ResultsA total of seven lactic acid bacteria strains (Z₁-Z₇) were isolated. Physiological and biochemical analysis showed that the Z₂ and Z₄ strains had low acid production capacity. Although Z₅ and Z₆ strains had strong acid production capacity, their growth rate was slow. Z₁, Z₃ and Z₇ strains have high acid production capacity and fast growth rate. Analysis of 16S rDNA sequence showed that the Z₁, Z₃ and Z₇ strains were all Lactobacillus plantarum, with a similarity of more than 99%.

ConclusionZ₁, Z₃ and Z₇ lactic acid bacteria have good silage potential and can be used as candidate strains for preparing silage additives.