Callus Induction and Adventitious Root Regeneration in Young Stem of Vaccinium dunalianum Wight

PurposeTo establish the genetic transformation system of Vaccinium dunalianum Wight, and to provide material reference for the production of important secondary metabolites such as arbutin and its derivatives in V. dunalianum.

MethodThe young stem segments of the tissue culture seedlings of V. dunalianum was taken as the explants, and the woody plant medium (WPM)was used in this study, the effects of different mass concentrations of 2,4-dichlorophenooxyacetic acid (2,4-D) and thidiazuron (TDZ) on callus induction and different cytokinins and mass concentrations on the differentiation of adventitious roots from callus were investigated in order to obtain the ideal formula of them.

ResultThe optimal medium formula for callus induction in the stem section of V. dunalianum was WPM+2,4-D 2.0 mg/L+TDZ 0.1 mg/L+sucrose 30 g/L+agar 5 g/L, the induced callus was milky white and firm, and the induction rate was up to 100%. In the stage of callus re-differentiation, WPM+TDZ 0.5 mg/L+sucrose 30 g/L+agar 5 g/L was the best formula for the adventitious roots induction, the induction rate of adventitious roots was up to 96.7%, and adventitious roots were numerous, long, orderly and homogeneous.

ConclusionThis study successfully established a system of callus induction from the young stems of V. dunalianum and callus differentiated adventitious roots, to provided a reference for the research on the production of important secondary metabolites and plant nutrition of V. dunalianum in the later reserch using callus and adventitious roots.