Transcriptome and Difference Analysis of Eocanthecona furcellata (Wolff)

PurposeEocanthecona furcellata (Wolff) is a natural predatory enemy widely distributed in tropical and subtropical regions, which is a potential biocontrol factor for many pests of Lepidoptera, Coleoptera and Hemiptera. Saliva plays a key role in the process of feeding. In order to understand the transcriptome characteristics and further study of gene expression in the salivary gland of E. furcellata.

MethodThe differential gene expression profiles of adult and third-instar nymph salivary glands were detected by Illumina sequencing technology, and the results were analyzed by clustering and functional annotation.

ResultA total of 26 022 sequences of salivary gland unigenes were obtained, with a mean length of 1 365 bp. By homologous sequence alignment search, 14 057 unigenes were annotated to the Nr, Nt, Pfam, Swiss-prot, GO, KOG and KEGG databases. Differential gene expression profiling revealed 3 437 differentially expressed genes, of which 1 871 unigenes were up-regulated and 1 566 unigenes were down-regulated in adult and third-instar nymph salivary glands, at the same time, three cathepsin B and three neuroendocrine protein 7b2 genes were identified.

ConclusionThis study enriched the molecular biology data of the Hemiptera, and provided some data support for the study of functional genes related to salivary secretion.