Distribution Diversity of Microbial Communities in the Air of Tea Gardens and Tea Cellars in Different Habitats of Jingmai Mountain, Yunnan Province

PurposeTo study the diversity and difference of air microbial community structure in tea gardens and tea cellars of different habitats in pu-erh tea area of Jingmai Mountain, so as to better ensure the consistency of microorganisms in pu-erh tea production in the original place of Jingmai Mountain.

MethodsAnderson air microbe sampler was used to sample the air of tea garden in four tea garden (CY-A- CY-D) and a tea cellar (CJ), plate coating separation technology was used to culture the collected microbe, and molecular biology technology was used to identify the species.

ResultsA total of 310 strains of bacteria and 191 strains of fungi were isolated and purified, respectively distributed in 27 bacterial genera and 18 fungal genera. The air microbial richness of the four tea gardens was different (CY-C>CY-A> CY-B>CY-D). Enterobacter was isolated from the four tea gardens, however, the microbial community of tea cellar was slightly different, excluding Enterobacter, and dominated by Streptomyces, Aspergillus and Bacillus.

ConclusionThe composition of air microbial community in different habitats of Jingmai Mountain is different. The more varieties of tea trees and intercropped plants, the more kinds of air microorganisms in tea garden. Enterobacter is a significant feature of the composition of air microbial communities in Jingmai Mountain tea area, which provides certain data support for further exploration of the source of pu-erh tea microorganisms.