Study on the Grape Rhizosphere Microbial Diversity in Shangri-La Wine Region by High-throughput Sequencing Technology

PurposeTo clarify the community diversity of rhizosphere microbes from different Cabernet sauvignon grown areas in Shangri-La, providing theoretical basis for screening and utilizing microorganism resources in Shangri-La region.

MethodsThe rhizosphere soils were collected from different vineyards of Shangri-La, soil microbial total DNA was extracted, the microbial community structure and diversity were analyzed by Illumina MiSeq high-throughput sequencing system.

ResultsThe results of rhizosphere soil microbial analysis showed that rhizosphere fungi could be classified into 131 genera in 6 phyla, and the predominant fungi included Ascomycete and Basidiomycete, rhizosphere bacteria could be classified into 50 phyla and 467 genera, among which Proteobacteria, Actinomycetes and Acidobacteria were the dominant phyla. In different vineyards of Shangri-La, the dominant fungi identified in soil were Fusarium, Laetisaria, Bionectria and Archaeorhizomyces; and the dominant bacteria were Rhodoplanes, Bacteroides, Nitrospira and Steroidobacter. However, the abundance of rhizosphere microbe was different among different vineyards. The abundance of fungi in soil sample from Dari vineyard increased significantly, including Fusarium, Bionectria, Chaetomium, Haematonectria, Rhizoctonia and Paecilomyces, while the abundance of bacteria in the soil samples from Benzilan vineyard was significantly increased, including Bacteroides, Rhodococcus, Phormidium, Blautia, Devosia and Bacillus. The results of redundancy analysis showed that soil conductivity, organic matter content, alkali-hydrolyzable nitrogen and available potassium had a great influence on rhizosphere bacterial community composition, but the effect of available phosphorus and pH value was not obvious.

ConclusionThe grape rhizosphere microbial diversity in the Shangri-La region is very rich, the fungi abundance in Dari vineyard was increased significantly, while the bacteria abundance in Benzilan vineyard was up-regulated significantly. The soil conductivity, organic matter content, alkali-hydrolyzable nitrogen and available potassium in different areas have great influence on rhizosphere bacterial community. The study of grape rhizosphere microbial diversity in Shangri-La could provide a theoretical basis for the utilization and development of characteristic microorganisms in this area.