Genetic Diversity of mtDNA D-loop Region in Tengchong White Chicken

PurposeIn order to clarify the genetic background information of Tengchong White Chicken.

MethodThe sequence variation of mtDNA D-loop region of 144 samples collected from Tengchong White Chicken was detected by direct sequencing of PCR products. On this basis, combined with published data from other indigenous chickens in Yunnan, the population genetic analysis was carried out.

ResultsIn the sequences of Tengchong White Chicken, the average contents of T, C, A and G were 30.2%, 29.8%, 27.3% and 12.6%, respectively. The average A+T content of the sequences was 57.6%. A total of 33 variable sites were detected, accounting for 6.35% of the total number of nucleotides detected, of which 29 were parsimonious information sites and 4 were single information sites. The main form of data mutation was transition. A total of 21 haplotypes were found in this population. Haplotype diversity (H), nucleotide diversity (π), average nucleotide difference between sequences and mean diversity in entire population were 0.890±0.011, 0.01641±0.00028, 8.534 and 0.017±0.004, respectively. The phylogenetic analysis showed that Tengchong White Chicken included 5 maternal lineages A, B, E, F and G, and the proportion of each lineage was 25.00%, 20.83%, 15.28%, 30.56% and 8.33%, respectively.

Conclution Tengchong White Chicken is rich in genetic diversity, which is different from other Yunnan indigenous chickens in the proportion of lineage composition, and has certain genetic differentiation with them.