Screening and Identification of Secretory Siderophore from Plant Pathogen

PurposeThe strain resources of high-yield siderophore were screened from different host plant pathogenic fungi, and using high-sorghum siderophore and pathogens to compete with Fe³⁺ can provide a new target for the control of plant diseases.

MethodsWe qualitatively and quantitatively screen the pathogenic strains of different host plants and the preliminary identification of siderophore types by CAS method and light absorption method, and biological identification of the dominant strains was according to 18S rDNA.

ResultsThere were 5 strains were identified as high-yield siderophore dominant strains from the test bacterial by the qualitative and quantitative screening, and the type of secretory siderophore are Hydroxamates, Carboxylates and Catecholates. According to the molecular biology identification, strain TZT-024 has similarity with Aspergillus fumigatus, strain TZT-033 is very similar to Botrytis cinerea, strain TZT-037 is analogous to Lecanicillium psalliotae, strain TZT-047 and TZT-069 respectively resemble Pythium pyrilobum and Pestalotiopsis vismiae.

ConclusionThere are no relevant researches of siderophore production about L. psalliotae, P. pyrilobum and P. vismiae, currently. Therefore, it can be used as a preferred material for the disease prevention by siderophore.