Amino Acid Sequence Analysis of Four Isolates of Hippeastrum Chlorotic Ringspot Virus Nucleocapsid Protein in Several Garden Plants in Kunming

PurposeThe amino acid sequences of HCRV nucleocapsid proteins in Kunming garden plants were compared and analyzed, to explore the viruses variation correlation between geographical distribution and host sources.

MethodIn 2016, samples of suspected Tospovirus virus were collected in the garden scenic spot of Kunming City, Yunnan Province. Four samples of HCRV infected with Zephyranthes candida, Crinum asiaticum, Philodendron schott and Oxalis corniculata were determined by RT-PCR. Four samples of S RNA sequences were found by cloning and sequencing and the nucleocapsid (N) protein was obtained using the online software ORF Finder and sequence analysis.

ResultC. asiaticum, P. schott and O. corniculata are the first reported new hosts of HCRV. The N protein of the four HCRV isolates and the first reported HLS1-2 N protein of NCBI identity reached 99.34%. The phylogenetic tree shows that the HCRV isolated from Yunnan in this study is clustered with the same strain from Yunnan published by GenBank.

ConclusionThe geographical distribution of the virus is stronger than the host correlation.