Nematicidal Activity against Caenorhabditis elegans of Goniothalamus cheliensis Hu

PurposeTo study the nematicidal activity against Caenorhabditis elegans of Goniothalamus cheliensis Hu.

MethodsThe extracts of methanol, water, dichloromethane, and ethyl acetate were obtained from stems and leaves of G. cheliensis. After 24, 48 and 72 h, the nematicidal activity of the above extracts with different concentration gradients against C. elegans were detected by using impregnation method.

ResultsThe extracts of dichloromethane and ethyl acetate from stems and leaves of G. cheliensis showed nematicidal activity against C. elegans, while water extract showed weak activity. After 72 h, the ethyl acetate extracts from stems and leaves showed better nematicidal activity with the adjust mortality of 69.9% and 70.3% at a mass concentration of 12.5 mg/mL, and of 80.2% and 80.4% at a mass concentration of 25 mg/mL, respectively; after 72 h, the ethyl acetate extracts from stems and leaves showed potential nematicidal activity with the adjust mortality ranged from 87.7% to 99.0% at a mass concentration of 50 mg/mL.

ConclusionsThe extracts of chloromethane and ethyl acetate from stems and leaves of G. cheliensis showed better nematicidal activity and it has the potentiality to further develop them as botanical pesticides.