In vitro Culture and Plant Regeneration of ‘Honglyu No.1’ Pepper

PurposeThe establishment of efficient regeneration system by in vitro culture of hot pepper can lay the foundation for its industrial seedling production.

MethodSterile explants were obtained from the seeds of ‘Honglyu No.1’ pepper from Yunnan Honglyu Capsaicin Co., Ltd. and the single-factor design and orthogonal design were employed to examine the effect of initiation medium, adventitious bud differentiation, proliferation, rooting culture and transplantation on this system.

ResultThe optimal initiation culture medium for adventitious bud induction was MS + 30 g/L sucrose + 7 g/L agar. MS + 2.5 mg/L 6-BA + 0.05 mg/L NAA + 5 mg/L AgNO₃ was optimal to adventitious bud differentiation and its differentiation rate was 93.3%. The bud elongation ratio was 100% when it was induced on MS + 5 mg/L 6-BA + 0.1 mg/L NAA. Rooting rate 100% of adventitious buds was obtained on B₅ + 30 g/L sucrose + 7 g/L agar. After seedling adaptation, the survival rate of regenerated plants was 98% when seedlings were transplanted into substrate.

ConclusionThe research established the rapid in vitro propagation system of ‘Honglyu No.1’.