Advanced Search
Changli MIN, Xuejun WANG, Yunjiang MIN, et al. Isolation, Screening and Antimicrobial Activities of Endophytic Actinomycetes in Ailanthus altissima Swingle[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2019, 34(6): 942-948. DOI: 10.12101/j.issn.1004-390X(n).201901030
Citation: Changli MIN, Xuejun WANG, Yunjiang MIN, et al. Isolation, Screening and Antimicrobial Activities of Endophytic Actinomycetes in Ailanthus altissima Swingle[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2019, 34(6): 942-948. DOI: 10.12101/j.issn.1004-390X(n).201901030
shu

Isolation, Screening and Antimicrobial Activities of Endophytic Actinomycetes in Ailanthus altissima Swingle

    Graphical Abstract
    • Abstract
  • PurposeTo isolate endophytic actinomycetes from Ailanthus altissima Swingle, and screen their antimicrobial activities in vitro.
    MethodEndophytic actinomycetes were isolated from the A. altissima by employing four selective media. Antimicrobial activities of these endophytic actinomycetes were determined by the agar diffusion method and tablet confrontation method, and the strain with high activity was identified by morphology observation and 16S rRNA sequence analysis.
    ResultThe best separating condition was HV medium. A total of 45 endophytic actinobacteria strains were isolated, 26 of them showed antimicrobial activity against the test strains which accounts for 57.78% of all strains. Among them, the strain AAA19 showed the highest antimicrobial activity. The identification result indicated that the strain AAA19 was Streptomyces antibioticus.
    ConclusionEndophytic actinomycetes isolated from A. altissima have antimicrobial activity, and the strain AAA19 has potential medicinal value.
    • FullText(HTML)
  • loading
    • References (27)
    • Related Articles

Catalog

    Turn off MathJax
    Article Contents
    Return