Preparation and Antiviral Activity of Kaempferia galanga L. Extract against Pseudorabies Virus in vitro

PurposeTo study the antiviral activity of extract of Kaempferia galanga L. against pseudorabies virus (PRV) in vitro.

MethodsResponse surface methodology was applied to optimize the ultrasonic extraction process of K. galanga; the mode of action and time of addition were determined by fluorescent quantitative polymerase chain reaction.

ResultsThe yield of total flavonoids was used as the response value of response surface methodology; the optimized extraction process was as follows: 80% ethanol, 208 W, and 98 min; the results of anti-PRV activity of the extract assay showed that 50% inhibition mass concentration of the extract was 55.85 μg/mL and the mode of action was through inhibition of viral penetration and replication; the addition of the extract within 24 h post PRV-infection could showed potent inhibitory effects.

ConclusionThe K. galanga extract exhibits significantly anti-PRV activity in vitro.